Zinc Colorimetric Assay Kit

WARNING: This product can expose you to chemicals including Trichloroacetic Acid, which is [are] known to the State of California to cause cancer.  For more information go to www.P65Warnings.ca.gov.

K387 is available from Abcam as ab102507.
Catalog #: K387 | abID: ab102507

Product Details

abID ab102507
Cat # +Size K387-100
Size 100 assays
Detection Method Absorbance (560 nm)
Species Reactivity Mammalian
Applications The kit can detect 1 µg/ml (7-15 µM)
Features & Benefits • Simple procedure; takes ~ 3 hours
• Fast and convenient
• The assay is sensitive and stable
• The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.
Kit Components • Zinc Reagent -1
• Zinc Reagent -2
• TCA (7%)
• Zinc Standard (50 mM
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Zinc, a metallic chemical element, symbol Zn and atomic number 30 is chemically similar to Magnesium due to its similar size and sole oxidation state of ²ᶧ. Zinc is an essential mineral of great biological significance, because many enzymes require it as an essential cofactor. Examples of zinc’s biological roles include signal transduction, gene expression, regulation of apoptosis, synaptic plasticity and prostate gland function. BioVision’s Zinc Assay Kit is a convenient colorimetric assay in which Zinc binds to a ligand with development of absorbance at 560 nm. The assay can be used with biological samples such as serum, plasma, csf or urine with detection sensitivity 0.2 µg/ml (~1-3 µM).

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Is the kit specific for Zinc?
K387 is based on binding of Zinc to a ligand to which other ions may also bind. But the non -specific binding is blocked by reagents in the kit making the reaction specific for Zinc ions.
What is the correlation between Zinc conc determined using this method and reference method such as Atomic Absorption Spectroscopy?
The correlation between the 2 methods is greater than 0.9 (>0.9).
I am working with plasma samples. Is it necessary to precipitate the samples with this kit? What is the different if I do not?
Yes, high protein containing samples like plasma samples need to be deproteinated. If this step is not done, you might see a lot of interference from enzymes and may end up getting inefficient results.
Can this kit be used with samples like bacteria, plants, drosophila, yeast etc?
We have optimized the kit with mammalian samples. However, theoretically these kits should work with samples from multiple species/sources. Since the optimal conditions depend on the sample type, the protocol has to be be adapted to fit the samples for efficient results. Please refer to this kit's citations to see what kind of samples have been used with this kit other than mammalian samples.
Can we use frozen samples with this assay?
Fresh samples are always preferred over frozen samples. However, frozen samples can also be used, provided, they were frozen right after isolation, were not freeze thawed multiple time (for which we recommend aliquoting the samples before freezing) and have been frozen for relatively short periods.
Can we use a different wavelength than recommended for the final analysis?
It is always recommended to use the exact recommended wavelength for the most efficient results. However, most plate readers have flexibility in their band width of detection in increments of +/- 10 nm. Depending on this flexibility range, you can deviate from the recommended wavelengths within limits.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
This kit is good for 12 months from the date of shipment in the unopened form when stored at the appropriate temperature and appropriate conditions. After opening and reconstitution, some of the components in this kit are good for 2 months at -20°C. Please refer to the datasheet for storage information and shelf life of each of the components.
Why are my standard curve values lower than those shown on the datasheet?
There are multiple factors which influence the signals like the incubation times, room temperature, handling etc. In general, to increase the value of the standards, you can increase the incubation time. As long as the standard curve is linear, it should be fine to use, since all of your samples will also be measured under the same conditions on this curve.
How do I normalize my samples against protein concentration
You can use a protein quantitation assay on the supernatants you get from cell/tissue lysates or with any other liquid sample in the assay buffer.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Should I make a standard curve for every expt I do, or is one curve/kit enough?
Yes, I would strongly recommend you to do the standards every time you do the expt. There is always a chance that something was done differently that day and we do not want any conditions to differ between standards and samples.
We are specifically measuring zinc levels in plasma and are wondering if TCA causes the zinc bound proteins (e.g. zinc bound to albumin) to be released to be measured by the assay? Or would the bound proteins be precipitated out with the TCA along with the zinc and zinc would not be measured?
When using TCA, protein is precipitated out from the samples and not the zinc. TCA precipitation of serum or plasma is the commonly used colorimetric method for detection of zinc. This kit measures free zinc ion in the samples and not zinc bound to proteins.
Kim, Gyuyoup et al. (2016) Transient Intermittent Hypoxia Exposure Disrupts Neonatal Bone Strength, Front Pediatr. 2016 Mar 7;4:15.
For more citations of this product click here