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ZEB1 (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of ZEB1 in human serum, plasma, tissue lysates, and other biological fluids

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E4998
$795.00

Product Details

Cat # +Size E4998-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA kit for quantitative measurement of ZEB1 in serum, plasma, cell and tissue lysates, and other biological fluids
Features & Benefits ● Sensitivity: 0.094 ng/ml
● Detection range: 0.156 – 10 ng/ml
● Recovery range: 85 - 105% for normal human serum and plasma samples
● This Sandwich ELISA is highly sensitive and highly specific for the detection of ZEB1 in human samples. There is no significant cross-reactivity or interference between ZEB1 and analogues
● Assay Precision: Intra-Assay CV and Inter-Assay CV < 10%
Kit Components ● Micro ELISA plate
● Wash Buffer (25X)
● Plate sealers
● Standard (Lyophilized) (10 ng)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Zinc finger E-box-binding homeobox 1 (ZEB1, TCF8) is a homeodomain transcription factor that belongs to the zinc finger family of proteins. ZEB1 is a master regulator of epithelial to mesenchymal transition; it binds to the promoter regions of epithelial marker proteins such as E-cadherin and inhibits their transcriptional activity, thereby promoting mesenchymal transition. ZEB1 also promotes differentiation of several tissues such as smooth muscles, bones, and neural tissues. Increased expression of ZEB1 has been reported in several cancers such as breast, lung, colon, liver, and pancreas, thereby making it an attractive target for anti-cancer therapy. BioVision’s ZEB1 (Human) ELISA kit quantitatively measures ZEB1 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of ZEB1 in the sample or standard.


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