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Product Details
| Kit Summary | BioVision’s XTT Cell Viability Assay Kit is a colorimetric assay for studying cell proliferation and cell cytotoxicity. |
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| Detection Method | Absorbance at 475 nm |
| Species Reactivity | Mammalian |
| Applications | ● Analysis of cytotoxic and cytostatic effects of drugs and toxins, antibodies and other exogenous chemicals. ● Measurement of cell viability and growth in response to growth factors, cytokines, mitogens and nutrients. ● Assessment of physiological mediators and antibodies that inhibit cell growth. |
| Features & Benefits | The kit provides a safe, easy-to-use, non-radioactive, high-throughput method for characterizing and screening cell viability and cytotoxicity. |
| Kit Components | ● XTT Reagent ● Electrocoupling Solution |
| Storage Conditions | -20ºC |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
BioVision’s XTT Cell Viability Assay Kit is a colorimetric assay for studying cell proliferation and cell cytotoxicity. The assay is based on the reduction of the XTT tetrazolium salt by NADH, which is produced by the mitochondrial dehydrogenase enzymes of metabolically active cells to generate an orange-colored formazan product in the cell media. This kit can be used for studying cell proliferation in response to cytokines, nutrients, growth factors and mitogens and also for analyzing the cytotoxic and cytostatic effects of drugs, toxins, antibodies and other exogenous chemicals. In this assay, the XTT working solution is added directly to the cell culture media and is read after an incubation of 0.5-4 hr. The measured absorbance at 475 nm is proportional to the number of viable cells. There are no steps in the assay involving changing of media or washing or addition of any solubilization reagents. The assay can detect cell numbers as high as 500,000 viable cells and as low as 1000 viable cells in each well. The presence of phenol red and/or serum in the cell growth media does not interfere with the assay. The kit provides a safe, easy-to-use, non-radioactive, high-throughput method for characterizing and screening cell viability and cytotoxicity.
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