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Xanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit

based on 3 citations in multiple journalsXanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit34.1 4
Catalog #: K710

In stock

$285.00

Product Details

Cat # +Size K710-100
Size 100 assays
Detection Method Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species Reactivity Mammalian
Applications The kit detects 1-100 mU xanthine oxidase in 100 µl reaction volume.
Features & Benefits • Simple procedure; takes ~ less than 40 minutes
• Fast and convenient
Kit Components • XO Assay Buffer
• OxiRed™ Probe
• DMSO (anhydrous)
• XO Enzyme Mix
• XO Substrate Mix
• XO Positive Control (~200mU)
• H₂O₂ Standard (0.88 M)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Xanthine oxidase (XO, EC 1.17.3.2 ) is present in appreciable amounts in the liver and jejunum in healthy individuals. However, in various liver disorders, XO is released into circulation. Therefore, determination of serum XO level serves as a sensitive indicator of acute liver damage such as jaundice. BioVision has developed an easy and sensitive assay to determine XO in variety of samples. In the assay, XO oxidizes xanthine to hydrogen peroxide (H₂O₂) which reacts stoichiometrically with OxiRed™ Probe to generate color (at λ = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to XO content, the XO activity can be accurately measured. The kit detects 1-100 mU xanthine oxidase in 100 µl reaction volume.


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How should blood be collected?
We recommend using heparin tubes.
Could you tell us the information about the each source of Enzyme Mix and XO Positive Control ?
K710 enzyme mix is from Horse Radish plant and the positive control is from buttermilk (food source).
I'd like to freeze my sample before use with this kit. Should I simply freeze the pellet, or is there a more appropriate step in the recommended sample preparation for this kit to freeze the samples?
The cell pellet after removing all media can be stored at -80C with a small volume of PBS. It is not ideal to store cells with medium. Once thawed, the cells can be washed once with PBS and then the PBS can be removed. At this step the assay buffer can be used for extraction according to the protocol.
Can we use frozen samples with this assay?
We would recommend using fresh samples. However, frozen samples can be used too provided they have not undergone multiple freeze thaw cycles.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6-12 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Ha, Y. et. al. Sigma Receptor 1 Modulates Endoplasmic Reticulum Stress in Retinal Neurons. Invest. Ophthalmol. Vis. Sci., Jan 2011; 52: 527 - 540.
Tual-Chalot, S. et al. Circulating Microparticles from Pulmonary Hypertensive rats Induce Endothelial Dysfunction. Am. J. Respir. Crit. Care Med., 2010; 182: 261-268.
Tual-Chalot, S. et al. Circulating Microparticles from Pulmonary Hypertensive Rats Induce Endothelial Dysfunction. Am. J. Respir. Crit. Care Med. 2010 10.1164/rccm.200909-1347OC
For more citations of this product click here
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