Xanthine/Hypoxanthine Assay Kit (ab155900)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Adherent cells, Cell culture supernatant, Milk, Other biological fluids, Plasma, Serum, Suspension cells, Tissue, Tissue Homogenate, Urine
- Sensitivity: 0.4 µM
Overview
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Product name
Xanthine/Hypoxanthine Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells, Tissue Homogenate -
Assay type
Enzyme activity -
Sensitivity
0.4 µM -
Assay time
1h 00m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Xanthine/Hypoxanthine Assay kit (ab155900) is a product where Xanthine/Hypoxanthine is specifically oxidized by the Xanthine Enzyme Mix to form an intermediate, which reacts with Developer & Probe to form a product that can be measured colorimetrically (λ = 570 nm) or fluorometrically (Ex/Em = 535/587 nm). Xanthine/Hypoxanthine Assay kit is rapid, simple and sensitive. This high-throughput suitable assay kit can detect Xanthine levels as low as 0.4 µM in various biological samples.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K685 Xanthine/Hypoxanthine Colorimetric/Fluorometric Assay Kit. K685-100 is the same size as the 100 test size of ab155900.
Xanthine, a catabolic product of purine metabolism, is present in body fluids, muscle tissue and certain plants. Structurally like caffeine, Xanthine has a stimulant effect and is used clinically to treat the congestive diseases such as asthma and chronic obstructive pulmonary disease. Xanthine is metabolized into uric acid and superoxide by Xanthine oxidase. Xanthine oxidase deficiency causes the rare genetic disorder-Xanthinuria, and leads to Xanthine accumulation in urine and blood, which ultimately progresses to renal failure. Recent studies show that Xanthine levels are elevated following ischemic injury, thus Xanthine can serve as a useful marker for tissue hypoxia. Early detection of Xanthine alteration in biological fluids is crucial for metabolic studies and for diagnostic and therapeutic monitoring.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer II 1 x 25ml Developer Solution V 1 vial Development Enzyme Mix VIII 1 vial OxiRed Probe 1 x 0.2ml Xanthine Standard 1 vial -
Research areas
Images
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Xanthine & Hypoxanthine measured in mouse tissue lysates by fluorimetric method showing quantity (nmol) per mg of sample tested.
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Xanthine & Hypoxanthine measured in biological fluids by fluorimetric method showing concentrations (micromolar).
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Xanthine & Hypoxanthine measured in mouse tissue lysates by colorimetric method showing quantity (mmol) per mg of sample tested.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab155900 has been referenced in 7 publications.
- Harris M et al. Ogfod1 deletion increases cardiac beta-alanine levels and protects mice against ischaemia- reperfusion injury. Cardiovasc Res 118:2847-2858 (2022). PubMed: 34668514
- Sedda D et al. Deletion of Mocos Induces Xanthinuria with Obstructive Nephropathy and Major Metabolic Disorders in Mice. Kidney360 2:1793-1806 (2021). PubMed: 35372998
- Lemaire F et al. Beneficial effects of the novel marine oxygen carrier M101 during cold preservation of rat and human pancreas. J Cell Mol Med 23:8025-8034 (2019). PubMed: 31602751
- Dervisevic M et al. Recent progress in nanomaterial-based electrochemical and optical sensors for hypoxanthine and xanthine. A review. Mikrochim Acta 186:749 (2019). PubMed: 31696297
- Brown AG et al. Exposure to intrauterine inflammation alters metabolomic profiles in the amniotic fluid, fetal and neonatal brain in the mouse. PLoS One 12:e0186656 (2017). PubMed: 29049352
- Weinstock NI et al. Metabolic profiling reveals biochemical pathways and potential biomarkers associated with the pathogenesis of Krabbe disease. J Neurosci Res 94:1094-107 (2016). PubMed: 27638595
- Zhou Y et al. Using Next-Generation Sequencing to Identify a Mutation in Human MCSU that is Responsible for Type II Xanthinuria. Cell Physiol Biochem 35:2412-21 (2015). PubMed: 25967871