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WST-NR-CV Combined Cytotoxicity Assay Kit

Sensitive, Non-radioactive Assay, measures cytotoxicity using 3 different methods
Catalog #: K543

Product Details

Cat # +Size K543-1000
Size 1000 assays
Kit Summary • Detection- Absorbance [OD: WST/NR/CV: 450 /540/570 nm
• Species reactivity- Mammalian
• Kit size- 1000
• Applications- The assay measures cell cytotoxicity in response to synthetic compounds and can be adapted for HTS applications
Detection Method Absorbance [OD: WST/NR/CV: 450 /540/570 nm
Species Reactivity Eukaryotes
Applications The assay measures cell cytotoxicity in response to synthetic compounds and can be adapted for HTS applications
Features & Benefits • Simple procedures; take less than 4 hours
• Convenient
• Uses a water-soluble tetrazolium salt WST reagent, Neutral Red and Crystal Violet . Method are simple and do not require extensive washing/harvesting/or solubilization steps. The entire assay can be performed directly in a 96-well plate.
Kit Components • WST Reagent (lyophilized)
• WST Developing Solution
• 100X NR Staining Solution
• 2X NR Solubilization Solution
• CV Staining Solution
• 10X CV Solubilization Solution
• 10X Washing Solution
• 20 mM Doxorubicin
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Cytotoxicity assays are essential tools in drug discovery. In vitro single parameter cytotoxicity assays, such as using tetrazolium salt (WST), neutral red (NR) or crystal violet (CV) in cultured cells, are widely used to assess the cytotoxicity of different compounds as these methods are fast, economical and animal-free. However, a major problem of using single parameter assays is determined IC50 data using these individual assays sometimes can be quite different due to specific compound inhibition. Consequently, if the cytotoxicity assay is not selected appropriately, false negative compounds that are actually cytotoxic may be easily missed. To partly overcome this problem, multiple parameter cytotoxicity assays are faster and more comprehensive alternatives to determine IC50 and cytotoxicity in different pathways. BioVision’s WST-NR-CV Combined Cytotoxicity Assay Kit can allow researchers to test the cytotoxicity of their compounds in different pathways such as in cell proliferation (WST), lysosome activity (NR) and total DNA synthesis (CV). The kit is simple and fast, yet it provides a quantitative measurement of the number of viable cells, it offers an excellent and efficient method for in vitro cytotoxicity studies as well as high-throughput drug screening.

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