A sensitive chromogenic indicator for NADH
Catalog #: 2198 | abID:

Product Details

Alternate Name 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt
Appearance Pale brown solid
CAS # 150849-52-8
Molecular Formula C₁₉H₁₁IN₅NaO₈S₂
Molecular Weight 651.35
Purity ≥97% by HPLC
Solubility H2O (10 mg/ml); 6.5 mg/ 10ml 50mM Tris buffer, pH 8.0
SMILES C1=CC(=CC=C1N2N=C(N=[N+]2C3=CC=C(C=C3)I)C4=C(C=C(C=C4)S(=O)(=O)[O-])S(=O)(=O)[O-])[N+](=O)[O-].[Na+]
InChi InChI=1S/C19H12IN5O8S2.Na/c20-12-1-3-13(4-2-12)23-21-19(22-24(23)14-5-7-15(8-6-14)25(26)27)17-10-9-16(34(28,29)30)11-18(17)35(31,32)33;/h1-11H,(H-,28,29,30,31,32,33);/q;+1/p-1
PubChem CID 6099081
MDL Number MFCD01075079
Handling Protect from air and light
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


A water-soluble tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH. It is reduced by NADH at neutral pHs in the presence of 1-methoxy PMS to produce the corresponding formazan dye that absorbs at 438 nM. Useful in cell proliferation assays as a cell viability indicator.

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Zhenzi Peng. Tumors exploit CXCR4hiCD62Llo aged neutrophils to facilitate metastatic spread. Oncoimmunology. Jan 2021. 33537175.