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Vitamin B12 (Vit-B12) ELISA Kit

A competitive ELISA kit for the quantitative measurement of Vitamin B12 in serum, plasma, tissue homogenates and other biological fluids

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to www.P65Warnings.ca.gov.
Catalog #: E4638
$645.00

Product Details

Cat # +Size E4638-100
Size 100 assays
Kit Summary • Detection method- Absorbance (450 nm)
• Species reactivity- Universal
• Application- quantitative measurement of Vitamin B12 in serum, plasma, tissue homogenates and other biological fluids.
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications This ELISA kit is used for quantitative measurement of Vitamin B12 in serum, plasma, tissue homogenates and other biological fluids.
Features & Benefits • Easy, convenient and time-saving method to measure the level of Vitamin B12 in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
• Detection Range: 0.781 - 50 ng/ml
• Sensitivity: < 0.469 ng/ml Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10% No significant cross-reactivity or interference was observed.
Kit Components • Micro ELISA Plate
• Lyophilized Standard
• Sample / Standard dilution buffer
• Biotin- detection antibody (Concentrated)
• Antibody dilution buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC dilution buffer
• TMB substrate
• Stop Solution
• Wash buffer (25X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Vitamin B12 (Vit-B12) is a water-soluble vitamin that is involved in the metabolism of every cell of the human body: it is a cofactor in DNA synthesis, and in both fatty acid and amino acid metabolism. It plays an important role in the synthesis of myelin, and in the maturation of developing red blood cells in the bone marrow. BioVision’s Vitamin B12 ELISA kit is a competitive ELISA assay for the quantitative measurement of Vit-B12 in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of Vit-B12 captured from the samples.


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Does this kit measure both bound and unbound Vitamin B12 in the sample? Specifically, if Vitamin B12 is bound to carrier protein in the serum or plasma, will it be detected by this assay?
Yes, this kit measures both bound and unbound Vitamin B12 in the sample.