Vitamin B12 ELISA Kit (ab287826)
Key features and details
- Sensitivity: 25 pg/ml
- Range: 50 pg/ml - 500 pg/ml
- Sample type: Other biological fluids, Plasma, Serum, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Quantitative
Overview
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Product name
Vitamin B12 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 8% Inter-assay Sample n Mean SD CV% Overall < 10% -
Sample type
Serum, Plasma, Other biological fluids, Tissue Homogenate -
Assay type
Quantitative -
Sensitivity
< 25 pg/ml -
Range
50 pg/ml - 500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 94 86% - 105% Hep Plasma 93 85% - 99% EDTA Plasma 98 92% - 105% -
Assay duration
Multiple steps standard assay -
Product overview
This Vitamin B12 ELISA kit ab287826 (previously known as Vitamin B12 ELISA kit, E4638) is a competitive ELISA assay for the quantitative measurement of Vit-B12 in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of Vit-B12 captured from the samples.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called E4638 Vitamin B12 (Vit-B12) ELISA Kit. E4638-100 is the same size as the 96 test size of ab287826.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests 96 tests 25X Wash buffer 1 x 30ml 1 x 30ml Antibody dilution buffer 1 x 10ml 1 x 10ml Biotin-Detection antibody (Lyophilized) 1 x 60µl 1 x 60µl HRP-Streptavidin Conjugate (SABC) 1 x 120µl 1 x 120µl Lyophilized Standard 2 vials 2 vials Micro ELISA Plate 1 unit 1 unit Plate Sealer 5 units 5 units SABC dilution buffer 1 x 10ml 1 x 10ml Sample and Standard Dilution Buffer 1 x 20ml 1 x 20ml Stop Solution 1 x 10ml 1 x 10ml TMB Substrate 1 x 10ml 1 x 10ml -
Research areas
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Relevance
Vitamin B12 is a member of the vitamin B complex. It contains cobalt, and so is also known as cobalamin. It is exclusively synthesised by bacteria and is found primarily in meat, eggs and dairy products. There has been considerable research into proposed plant sources of vitamin B12. Fermented soya products, seaweeds, and algae such as spirulina have all been suggested as containing significant B12. However, the present consensus is that any B12 present in plant foods is likely to be unavailable to humans and so these foods should not be relied upon as safe sources. Vitamin B12 is necessary for the synthesis of red blood cells, the maintenance of the nervous system, and growth and development in children. Deficiency can cause anaemia. Vitamin B12 neuropathy, involving the degeneration of nerve fibres and irreversible neurological damage, can also occur. -
Cellular localization
Secreted -
Alternative names
- Alpha (5 6 Dimethylbenzimidazolyl)cyanocobamide
- Cyanocobalamin
Images
Datasheets and documents
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Datasheet download
References (0)
ab287826 has not yet been referenced specifically in any publications.