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Urokinase Inhibitor Screening Kit (Fluorometric)

Simple Assay to Screen Urokinase Inhibitors
Catalog #: K727
$435.00

Product Details

Cat # +Size K727-100
Size 100 assays
Detection Method Fluorescence (Ex/Em = 350/450 nm)
Applications Screening potential inhibitors of Urokinase
Characterize/study urokinase inhibitors
Features & Benefits • Rapid & simple
• Reliable test for screening potential inhibitors of Urokinase
• Includes Urokinase-specific Inhibitor [GGACK Dihydrochloride]
Kit Components • Urokinase Assay Buffer
• Urokinase Substrate
• Human Urokinase
• Urokinase Inhibitor [GGACK Dihydrochloride] (10 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Urokinase (Urokinase-type plasminogen activator, uPA, EC 3.4.21.73) is a serine protease, which catalyzes the conversion of the plasminogen to active plasmin, which in turn participates in thrombolysis. Urokinase inhibitors have potential applications in cancer therapy. Biovision’s Urokinase Inhibitor Screening Kit utilizes the ability of Urokinase to cleave a synthetic AMC-based peptide substrate to release AMC, which can be detected by measuring its fluorescence at Ex/Em = 350/450 nm. In the presence of the Urokinase specific inhibitors, the enzyme activity is inhibited or completely abolished. This assay kit is a simple, sensitive, and rapid tool to screen potential Urokinase inhibitors.


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Can we use frozen samples with this assay?
We would recommend using fresh samples. However, frozen samples can be used too provided they have not undergone multiple freeze thaw cycles.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6-12 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.