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Uricase Activity Assay Kit (Fluorometric)

Simple Assay, HTS
Catalog #: K734

Product Details

Cat # +Size K734-100
Size 100 assays
Kit Summary • Detection method-- Fluorometric (535/587 nm)
• Species reactivity- Mammalian/Plants
• Applications- The kit can detect as low as 10 µU of Uricase
Detection Method Detection method-- Fluorometric (535/587 nm)
Species Reactivity Eukaryotes
Applications Rapid assessment of Uricase activity in mammalian and plant tissues
Features & Benefits • Simple procedure; takes ~ 1 hour
• Fast and convenient
• The assay is sensitive, stable and high-throughput adaptable.
Kit Components • Uricase Assay Buffer
• Uricase Substrate
• Uricase Probe (in DMSO)
• Uricase Enzyme Mix
• H2O2 Standard (0.88 M)
• Uricase Positive Control
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Uricase (factor-independent urate hydroxylase, urate oxidase (UO); EC is an enzyme involved in the uric acid metabolism. Uric acid is the end product of purine metabolism, and high levels of uric acid in blood causes gout. Uricase is present in a wide range of mammals but absent from human beings. It has been widely used for the measurement of uric acid concentrations in biological samples and recombinant uricase (rasburicase) has been used as a drug for the prevention of tumor lysis syndrome. BioVision’s Uricase Activity Assay Kit provides a quick and easy method for the measurement of uricase activity in a wide variety of samples. In this assay, uricase oxidizes uric acid forming a product that reacts with the probe, thus generating a fluorescence signal (Ex/Em= 535/587nm). The generated fluorescence signal is directly proportional to the amount of active uricase present in the sample. This kit provides a sensitive and high throughput adaptable assay and can measure uricase activity in biological samples as low as 10 µU.

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