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UbcH5a, human recombinant (His-tag)

An essential ubiquitin-conjugating enzyme
Catalog #: 4851
SKU-Size Size Price Qty
4851-10 10 μg
4851-100 100 μg
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Product Details

Alternate Name UbcH5a, Ubiquitin conjugating enzyme H5a, Ubiquitin conjugating enzyme, Ubiquitin-conjugating enzyme E2 D1, Stimulator of Fe transport, UBC4/5 homolog, UbcH5, Ubiquitin carrier protein D1, Ubiquitin-conjugating enzyme E2(17)KB 1, Ubiquitin-conjugating enz
Gene Symbol UB2D1
Gene ID 7321
Accession # P51668
Source E. coli
Appearance Liquid
Physical Form Description Supplied as a liquid in 20 mM Tris-HCl, pH 8, containing 0.5 mM DTT.
Molecular Weight 17.0 kDa
Purity by SDS-PAGE ≥95%
Handling Centrifuge the vial prior to opening.
Storage Conditions -80°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not to be used in humans


UbcH5a is highly homologous to the S. cerevisiae Ubc4/5 family of E2 enzymes demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5a, together with UbcH5b and UbcH5c, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors. Specifically, UbcH5a stimulates the conjugation of ubiquitin to the tumor suppressor p532. Useful for in vitro ubiquitinylation reactions. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 µM depending upon conditions.

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