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UbcH1, human recombinant (GST-tag)

An essential ubiquitin-conjugating enzyme
Catalog #: 4846
SKU-Size Size Price Qty
4846-10 10 μg
$145.00
4846-100 100 μg
$350.00
More Sizes Get Quote

Product Details

Alternate Name UbcH1, Ubiquitin conjugating enzyme 1, Ubiquitin conjugating enzyme
Accession # P61086
Source E. coli
Appearance Liquid
Physical Form Description Supplied at a concentration of 1.0 mg/ml in 20 mM TBS, pH 7.5 containing 1.0 mM DTT.
Molecular Weight 22.4 kDa
Purity by SDS-PAGE ≥95%
Handling Centrifuge the vial prior to opening.
Storage Conditions -80°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not to be used in humans

Details

Among ubiquitin-conjugating enzymes, the mammalian ubiquitin conjugating enzyme UbcH1, is unique in its ability to catalyze the in vitro synthesis of unanchored Lys48-linked poly-ubiquitin chains from mono- or poly-ubiquitin, E1, and ATP. In addition, UbcH1 can catalyze the cyclization of longer poly-ubiquitin chains, including tetra- and penta-ubiquitin. Recombinant UbcH1 charges and supports ubiquitinylation in vitro. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 µM.


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