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Tylosin ELISA Kit

A Competitive ELISA kit for in vitro quantitative determination of Tylosin concentrations in Tissue and Honey samples

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to www.P65Warnings.ca.gov.
Catalog #: E4779
$595.00

Product Details

Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications in vitro quantitative determination of Tylosin concentrations in Tissue and Honey samples
Features & Benefits • Sensitivity: 0.5 ppb (ng/mL)
• Detection limit: Tissue (muscle) - 1 ppb, Honey - 0.5 ppb
• Cross-reactivity: Tylosin - 100%, Erythromycin - 1%, Other macrolides <1%
• Sample recovery rate: Tissue - 85%±10%, Honey- 85%±15%
Kit Components • HRP Conjugate
• ELISA plate
• Plate Sealer
• Reconstitution Buffer (2X)
• Substrate Reagent B
• Antibody Working Solution
• Standard
• Stop Solution
• Substrate Reagent A
• Wash Buffer (20X)
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Tylosin is an antibiotic and a bacteriostatic feed additive used in veterinary medicine. It has a broad spectrum of activity against Gram-positive organisms and a limited range of Gram-negative organisms. It is found naturally as a fermentation product of Streptomyces fradiae. It is a macrolide antibiotic. Tylosin is used in veterinary medicine to treat bacterial infections in a wide range of species and has a high margin of safety. It has also been used as a growth promotant in some species, and as a treatment for colitis in companion animals. Tylosin ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with Tylosin. During the reaction, Tylosin in the samples or standard competes with Tylosin coated on the plate for binding to the anti-Tylosin antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Tylosin. The concentration of Tylosin in the samples can be calculated by comparing the OD of the samples to the standard curve.


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