TSLP (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of TSLP in human plasma, tissue lysates, and other biological fluids


WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.p65warnings.ca.gov/
Catalog #: E4939 | abID:

Product Details

Cat # +Size E4939-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This Sandwich ELISA kit is designed to quantitatively measure amount of TSLP in human serum, plasma, tissue lysates, and other biological fluids
Features & Benefits • Detection range: 31.25 - 2000 pg/ml
• Sensitivity: 18.75 pg/ml
• Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
• This Sandwich ELISA is highly sensitive and highly specific for the detection of TSLP in human samples. There is no significant cross-reactivity or interference between TSLP and analogues
• Recovery range: 85 - 105% for normal human serum and plasma samples
Kit Components • Micro ELISA plate
• Wash Buffer (25X)
• Plate Sealers
• Standard (Lyophilized) (2000 pg)
• Sample/Standard Dilution Buffer
• Biotin-labeled Antibody
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate Solution
• Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Thymic stromal lymphopoietin (TSLP) is a cytokine that belongs to the four-helix-bundle cytokine family and a distant paralog of cytokine IL-7. Similar to IL-7, TSLP can also stimulate thymocytes and induce B cell lymphopoiesis. Cells that produce TSLP include epithelial cells, keratinocytes, dendritic cells, mast cells, and stromal cells. TSLP plays an important role in developing responses to allergens and the pathogenesis of type 2 inflammatory diseases such as atopic dermatitis, food allergies, and asthma. Recent studies have demonstrated the role of TSLP beyond allergic inflammation; in cancer (breast and prostate) and autoimmune diseases such as rheumatoid arthritis, inflammatory bowel disease (IBD), and multiple sclerosis. Due to its versatile role in multiple diseases, TSLP is considered an attractive therapeutic target. BioVision’s TSLP (Human) ELISA kit is based on the Sandwich principle to quantitatively measure the concentration of TSLP in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the concentration of TSLP in the sample or standard.


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