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New

Pepsin/Pepsinogen Activity Assay Kit (Fluorometric)

Highly sensitive and specific
Catalog #: K446

In stock

$365.00

Product Details

Cat # +Size K446-100
Size 100 assays
Kit Summary • Detection method- Fluorescence (Ex/Em: 328/418 nm)
• Species reactivity- Mammalian
• Application- The kit detects as low as 500 µU pepsin in various samples.
Detection Method Fluorescence (Ex/Em 328/418 nm)
Species Reactivity Mammalian
Applications Estimation of pepsin activity in various biological samples
Features & Benefits • Simple procedure; takes ~ 1-2 hours based on pepsin levels
• Fast and convenient
Kit Components • Pepsin Assay Buffer
• Pepsin Substrate
• Pepsin Inhibitor (Pepstatin A)
• Pepsin Positive Control
• MCA Standard
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Pepsin (EC 3.4.23.1) is a peptidase A1 family aspartic protease that serves as a primary digestive enzyme in the mammalian stomach. Pepsin is synthesized in the chief cells of the gastric mucosa as the inactive pro-enzyme pepsinogen, which contains a 44 amino acid autoinhibitory domain. The proenzyme is packaged into granules and stored inside the gastric mucosal cells. When food is consumed, neuronal signals traveling along the vagus nerve trigger secretion of HCl and pepsinogen into the stomach. In the highly acidic environment of the stomach, pepsinogen unfolds and undergoes autocatalytic cleavage, forming the active protease pepsin. Infection by the bacterium H. pylori induces hypersecretion of both stomach acid and pepsin, contributing to the formation of gastric and duodenal ulcers. In addition, excessive amounts of pepsin can play a role in the degradation of the esophageal and laryngeal tissue observed in chronic gastroesophageal reflux disease. While the majority of pepsinogen is expressed in the gastric mucosa, a small amount of the inactive enzyme is also released into the blood. Serum pepsinogen levels correlate with functional changes to gastric mucosa and are often measured for the screening of gastric cancer and chronic H. pylori-induced gastritis. BioVision’s Pepsin Assay Kit is a homogenous assay that allows for quantification of pepsin activity in gastric tissue and various biological fluids. The assay utilizes a synthetic peptide substrate bearing both a fluorophore and a fluorescence quencher. Upon cleavage by pepsin, the fluorophore-bearing peptide fragment is unquenched to produce a bright fluorescent signal (Ex/Em = 328/418 nm). Lysosomal aspartic proteases in the peptidase A1 family (Cathepsin D and E) do not interfere with the assay. The assay is rapid, simple to perform and is vastly more sensitive than the classical hemoglobin degradation assay, with a detection limit of 500 μU pepsin activity per well.


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