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Trypsin Activity Assay Kit (Fluorometric)

To detect trypsin activity
Catalog #: K2023

Product Details

Cat # +Size K2023-100
Size 100 assays
Detection Method Fluorescence (Ex/Em = 380/460 nm)
Species Reactivity All
Applications Measurement of trypsin activity in Biological Fluids, Tissues
Features & Benefits • Can detect as low as 2 µU of trypsin activity in samples
• Specific and sensitive
• Rapid way to detect trypsin activity in a wide variety of biological samples
• Simple
Kit Components • Trypsin Inhibitor
• Trypsin Substrate
• Trypsin Assay Buffer
• AMC Standard
• Positive Control (lyophilized)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Trypsin (EC is a serine protease that is expressed in bacteria, fungi and mammals. Trypsin cleaves peptide chains at the carboxyl side of the amino acids namely arginine and lysine. In mammals, trypsin is secreted from the pancreas as an inactive precursor (trypsinogen) and is processed to its active form in the small intestine, where it plays an essential role in protein hydrolysis and absorption. Activation of trypsin in pancreas can lead to a series of events thereby resulting in pancreatitis. Trypsin is therefore a useful biomarker for pancreatitis. Additionally, monitoring trypsin activity plays a central role in biochemical, pharmaceutical and has clinical applications. BioVision’s Trypsin Activity Assay Kit provides a simple, rapid way to detect trypsin activity in a wide variety of biological samples. In this kit, trypsin cleaves a synthetic substrate thereby releasing a fluorophore (AMC), which can be easily quantified at Ex/Em = 380/460 nm. By pretreating samples at 60ºC for 20 min, the kit can differentiate the trypsin activity from other structurally similar enzymes such as chymotrypsin and tryptase. The assay is specific, sensitive and can detect as low as 2 µU of trypsin activity in samples.

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How does heating the samples at 60°C makes the assay specific for Trypsin and differentiate from Chymotrypsin and Tryptase?
The substrate used in the kit has a very high affinity for Trypsin and some affinity towards Chymotrypsin and Tryptase. This affinity is pH and temperature dependent. At 60°C and pH 8, the substrate is highly specific to Trypsin and Chymotrypsin and Tryptase shows no activity. The pH is adjusted to pH 8 in the Assay Buffer.