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Trimethoprim ELISA Kit

A Competitive ELISA kit for quantitative measurement of Trimethoprim in tissue, urine, and serum samples
Catalog #: E4947
$725.00

Product Details

Cat # +Size E4947-100
Size 96 assays
Kit Summary Trimethoprim is an antibiotic that targets bacterial dihydrofolate reductase, an enzyme responsible for catalyzing the reduction of dihydrofolic acid to tetrahydrofolic acid. Inhibition of DHFR prevents bacterial DNA synthesis and subsequent bacterial survival. The drug is commonly used in combination with sulfamethoxazole due to synergistic effects and the potential to decrease drug resistance. Trimethoprim is primarily used to treat urinary tract infections but is also used to treat middle ear infections, pneumonia, and traveler’s diarrhea. BioVision’s Trimethoprim ELISA kit is used to quantitatively measure Trimethoprim in muscle, kidney and liver tissue, feed, serum, and urine samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Trimethoprim antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Trimethoprim in the samples.
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications Competitive ELISA to quantitatively measure Trimethoprim in tissue, serum and urine samples
Features & Benefits • This ELISA kit is used for in vitro quantitative determination of Trimethoprim Sensitivity: 0.03ppb
• Cross reaction: Trimethoprim 100%, Sulfapyridine, Sulfamethoxazole, Sulfisoxazole, Sulfathiazole, Sulfamerazine, Sulfadoxine – all<0.1%
• Detection Limit: 1.6ppb for feed, 0.4ppb for tissues (muscle, kidney, liver), 0.4ppb for serum, urine
Kit Components • Micro ELISA Plate Standard (S0 – S5)
• HRP Conjugate
• Substrate A
• Substrate B
• Stop Solution
• Wash Buffer (20X)
• Reconstitution Buffer (2X)
• Plate Sealer
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Trimethoprim is an antibiotic that targets bacterial dihydrofolate reductase, an enzyme responsible for catalyzing the reduction of dihydrofolic acid to tetrahydrofolic acid. Inhibition of DHFR prevents bacterial DNA synthesis and subsequent bacterial survival. The drug is commonly used in combination with sulfamethoxazole due to synergistic effects and the potential to decrease drug resistance. Trimethoprim is primarily used to treat urinary tract infections but is also used to treat middle ear infections, pneumonia, and traveler’s diarrhea. BioVision’s Trimethoprim ELISA kit is used to quantitatively measure Trimethoprim in muscle, kidney and liver tissue, feed, serum, and urine samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Trimethoprim antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Trimethoprim in the samples.


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