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TPA, Human Recombinant (Sf9)

Plays a role in cell migration and tissue remodeling.
Catalog #: P1325

In stock

SKU-Size Size Price Qty
P1325-5 5 µg
$295.00
P1325-50 50 µg
$1,995.00
More Sizes Get Quote

Product Details

Alternate Name Tissue-type plasminogen activator, EC 3.4.21.68, tPA, t-PA, t-plasminogen activator, TPA, T-PA, DKFZp686I03148, PLAT and tPA.
Gene Symbol PLAT
Accession # P00750
Source Sf9, Baculovirus cells
Appearance Sterile Filtered colorless solution
Physical Form Description tPA protein solution (0.25 mg/ml) containing 50 mM MES buffer(pH 5.5 ), 40% glycerol, 5 mM CaCl2, 1mM DTT and 0.5 M NaCl.
Molecular Weight 61.3 kDa
Purity by SDS-PAGE ≥ 90%
Reconstitution Instructions Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time and for long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Amino Acid Sequence QEIHARFRRG ARSYQVICRD EKTQMIYQQH QSWLRPVLRS NRVEYCWCNS GRAQCHSVPV KSCSEPRCFN GGTCQQALYF SDFVCQCPEG FAGKCCEIDT RATCYEDQGI SYRGTWSTAE SGAECTNWNS SALAQKPYSG RRPDAIRLGL GNHNYCRNPD RDSKPWCYVF KAGKYSSEFC STPACSEGNS DCYFGNGSAY RGTHSLTESG ASCLPWNSMI LIGKVYTAQN PS
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

Tissue plasminogen activator (PLAT or tPA) is a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Plasminogen is synthesized as a single chain which is cleaved by PLAT into the two chain disulfide linked plasmin. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. tPA Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 545 amino acids (24-562 a.a) and having a molecular mass of 61.3 kDa (Molecular size on SDS-PAGE will appear at approximately 50-70 kDa). tPA is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.


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