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TPA (311-562), Human Recombinant

Plays a role in cell migration and tissue remodeling.
Catalog #: P1322

In stock

SKU-Size Size Price Qty
P1322-10 10 µg
$295.00
P1322-1000 1 mg
$7,995.00
More Sizes Get Quote

Product Details

Alternate Name Tissue-type plasminogen activator, EC 3.4.21.68, tPA, t-PA, t-plasminogen activator, TPA, T-PA, DKFZp686I03148, PLAT
Gene Symbol PLAT
Accession # P00750
Source Sf9, Baculovirus cells
Appearance Sterile Filtered colorless solution
Physical Form Description TPA protein solution (0.5mg/ml) contains 50mM MES (pH 5.0), 5mM CaCl2, 1mM DTT, 0.5M NaCl and 30% glycerol.
Molecular Weight 29.2 kDa
Purity by SDS-PAGE ≥ 95%
Reconstitution Instructions Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time and for long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Amino Acid Sequence ADPIKGGLFA DIASHPWQAA IFAKHRRSPG ERFLCGGILI SSCWILSAAH CFQERFPPHH LTVILGRTYR VVPGEEEQKF EVEKYIVHKE FDDDTYDNDI ALLQLKSDSS RCAQESSVVR TVCLPPADLQ LPDWTECELS GYGKHEALSP FYSERLKEAH VRLYPSSRCT SQHLLNRTVT DNMLCAGDTR SGGPQANLHD ACQGDSGGPL VCLNDGRMTL VGIISWGLGC GQ
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

Tissue plasminogen activator (PLAT or tPA) is a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Plasminogen is synthesized as a single chain which is cleaved by PLAT into the two chain disulfide linked plasmin. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. TPA Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 261 amino acids (311-562 a.a.) and having a molecular mass of 29.2 kDa (Migrates at 28-40 kDa on SDS-PAGE under reducing conditions). TPA is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.


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