Product Details
| Cat # +Size | K2507-10 |
|---|---|
| Size | 10 columns |
| Species Reactivity | All |
| Applications | • Ideal for processing small scale solution samples (0.1 ml - 0.5 ml) • Effectively eliminate endotoxins to < 0.05 EU/ml |
| Features & Benefits | • Simple and fast procedure: Takes less than one hour to get endotoxin-free sample • Final products are suitable for potential downstream therapeutic applications as ligand and buffers are nontoxic • High Capacity: up to 99.99% endotoxin can be removed in a single extraction at 1E+7 EU/ml • One kit can clean up to 5 ml protein sample at 1-5 mg/ml of concentration • Very low residual detergent: < 1 µg/ml • Lower endotoxin level to < 0.05 EU/ml in a single extraction from up to 5,000 EU/ml sample • High Sample Recovery: >90% recovery with protein solution samples (typically > 95%) |
| Kit Components | • ToxOutTM Phase Extraction Endotoxin Removal Reagent A • ToxOutTM Phase Extraction Endotoxin Removal Reagent B • ToxOutTM Phase Separation Indicator • ToxOutTM Triton X-114 cleaning column • ToxOutTM Endotoxin-free Collection Tube |
| Storage Conditions | 4°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Endotoxin is a lipopolysaccharide (LPS) complex located in the outer membrane of gram-negative bacteria. A single E.coli bacterium contains ~2 million LPS molecules. During experimental procedures, large amount of endotoxin is shed and can easily contaminate labware, buffers and downstream products. In vitro, endotoxin causes a variety of problems in cell-based research. In vivo, endotoxin may cause various side effects, including inflammatory response, organ failure or septic shock in host organisms. Therefore, it is critical to remove endotoxin from samples and products. BioVision‘s ToxOutTM Phase Extraction Endotoxin Removal Kit can effectively eliminate high concentration endotoxin in solutions containing proteins or pharmacologically important components down to <0.05 EU/ml without using toxic buffers, while maintaining protein recovery at >90%. BioVision‘s ToxOutTM Phase Extraction Endotoxin Removal Kit is based on Triton X-114 Phase Extraction (PE), which shows strong endotoxin removal capabilities. The residual Triton X-114 is further eliminated with our proprietary beads to achieve minimal detergent level in the final samples. The protein recovery is >90% depending on individual protein and typically greater than 95%.
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