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Total Sulfite Assay Kit (Colorimetric)

Highly sensitive, quick, colorimetric assay to measure total sulfite.
Catalog #: K699

Product Details

Cat # +Size K699-100
Size 100 assays
Detection Method Absorbance (570 nm)
Applications Estimation of sulfite in various food and beverage samples.
Features & Benefits • Highly sensitive, quick, colorimetric assay.
• Can detect as low as 20 µM of sulfite in a variety of samples.
Kit Components • Sulphite Assay Buffer
• Sulphite Stabilizer
• Sulphite Probe
• Sulphite Oxidizing Mix
• Sulphite Enzyme Mix
• Sulphite Standard
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Sulfites/sulphites are substances that occur naturally in the human body due to the metabolism of amino acids containing sulfur in their side chains. They are readily oxidized to sulfates via enzymatic reactions and excreted in urine at the rate of 1000 mg/day. Sulfites are often considered as allergens due to adverse symptoms observed in asthmatic patients. Exogenous sources of sulphites include polluted air, food and beverages containing sulphur dioxide (SO2). SO2 is a molecule that easily reacts with several small compounds including aldehydes, ketones, anthocyanins, cobalamine, thiamine, NAD, flavins, among others. SO2 also interacts with cysteine residues in proteins and promotes cross-linking. Sulfites are also used as regulated food preservatives/additives/enhancers in dried fruits, wine, beer, etc. They are considered as GRAS (Generally Recognized as Safe) in food and beverages. In wine, SO2 reacts with sugars, aldehydes and anthocyanins. The term sulfite is used for all sulfite-derived molecules: bisulfites, metasulfites, and SO2. Total sulfite is defined as the sum of bound and free sulfite. BioVision’s Total Sulfite Assay kit is a simple and sensitive assay to detect small concentrations of sulfites in a variety of food and beverage samples. This assay is based on the oxidation of sulfite to sulfate producing a stable signal at 570 nm, which is directly proportional to the amount of sulfite in the sample. This assay is very sensitive and can detect as low as 20 µM of sulfite in a variety of samples.

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Can we use frozen samples with this assay?
We would recommend using fresh samples. However, frozen samples can be used too provided they have not undergone multiple freeze thaw cycles.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6-12 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Do you know if infant formula can be used as a sample? Have you ever tested infant formula?
We have not tested infant formula samples using K699. However, K699 can be used to test dairy products. Therefore, it is expected to work with infant formula samples. The end user can prepare samples in the Assay Buffer provided with the kit.