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Total Collagen Assay Kit (Perchlorate-Free)

A simple, sensitive & easy colorimetric assay to quantitate total collagen levels.
Catalog #: K406

Product Details

Cat # +Size K406-100
Size 100 Reactions
Kit Summary • Detection method- Absorbance (OD = 560 nm).
• Sample Types- Mammalian tissues, protein/peptide hydrolysates, serum, Urine
• Applications- Measurement of collagen in various sample types.
Detection Method Detection method- Absorbance (OD = 560 nm).
Species Reactivity Mammalian tissues, protein/peptide hydrolysates, serum, urine
Applications Measurement of collagen in various sample types.
Features & Benefits • Simple, fast and convenient assay
• Can detect as low as 0.5 µg of collagen in a variety of samples
• Simple procedure; takes less than ~60 minutes
• Faster than Perchlorate-Based Assay
• PCA-free
Kit Components • Oxidation Buffer
• Chloramine T Concentrate
• Developer Solution
• DMAB Concentrate (in DMSO)
• Collagen I Standard (3 mg/ml)
• Microplate Sealing Film
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Collagen is the most abundant protein in mammals, accounting for nearly 30% of body protein content. Collagen is an extracellular scaffolding protein found in skin, tendons, bone, cartilage, ligaments and muscles, amongst other tissues. There are more than twenty different characterized types of collagen, but the fibrilous types I, II and III comprise more than 90% of the total collagen in mammals. Collagen has a triple helical structure, consisting of three ‘preprocollagen’ strands with a repeating Gly-X-Hyp tripeptide sequence, where X is any amino acid and Hyp is hydroxyproline, a post-translationally modified amino acid formed from proline by the enzyme prolyl-4-hydroxylase. In animals, hydroxyproline is found almost entirely in collagen (which contains approximately 12-14% hydroxyproline by mass) and contributes to its structural rigidity and high tensile strength. Hydroxyproline is thus a direct measure of the amount of collagen present in tissues. Many diseases are believed to affect collagen turnover, including tumor invasion and metastasis, rheumatoid arthritis, cardiopulmonary fibrosis and muscular dystrophy. BioVision’s Total Collagen Assay Kit II (Perchlorate-Free) is a quick and convenient protocol for detection of small amounts of collagen in a variety of biological samples. The assay is based upon hydrolysis of samples to yield free Hyp, which is oxidized and reacted to give a brightly-colored chromophore with an absorbance peak at 560 nm. The assay employs a proprietary acidic developer solution to accurately measure collagen in hydrolysates without the use of hazardous perchloric acid, obviating the need for any special handling and waste-disposal protocols. The assay can detect a minimum of 0.5 µg collagen per well in a 96-well format and generates colorimetric results that are virtually identical to those of the classical perchloric acid-based method

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In the protocol, it is recommended to use vials with screwcaps (BV Cat# M1352 and M1353). However, we want to set the assay on a 96-well plate. Do you know what type of plate should we use that would be equivalent to these tubes?
It is not possible to use 96 well plates for the hydrolysis step as you need to do it in a tightly sealed tube to avoid evaporation during high temperature.