Total Carbohydrate Assay Kit - Quantification (ab155891)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Adherent cells, Suspension cells, Tissue, Tissue Extracts
Overview
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Product name
Total Carbohydrate Assay Kit - Quantification
See all Carbohydrate kits -
Detection method
Colorimetric -
Sample type
Tissue, Adherent cells, Suspension cells, Tissue Extracts -
Assay type
Quantitative -
Assay time
0h 30m -
Product overview
Total Carbohydrate Assay (ab155891) is a simple, sensitive and robust method of detecting virtually all carbohydrates.
The carbohydrate assay protocol is based on the phenol - sulfuric acid assay. In the assay, polysaccharides (mono, di, tri, etc.) and their derivatives, in the presence of sulfuric acid, are hydrolyzed to monomers and converted to furfural or hydroxyfurfural. These react with the developer to form a chromogen that can be quantified by measuring the absorbance at λ = 490 nm.
The total carbohydrate assay kit can detect most forms of carbohydrates, including simple and complex saccharides, glycans, glycoproteins and glycolipids.
Total carbohydrate assay protocol summary:
- add samples and glucose standards to wells
- add concentrated sulfuric acid and incubate for 15 min at 90ºC
- add developer and incubate for 5 min
- analyze with a microplate reader -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K645 Total Carbohydrate Colorimetric Assay Kit. K645-100 is the same size as the 100 test size of ab155891.
Carbohydrates play important structural as well as chemical roles in all living systems. Detection of total carbohydrates, therefore, has wide applications.
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Platform
Microplate reader
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests Assay Buffer VII 1 x 25ml Developer Solution IV 1 x 3ml D-Glucose Standard 1 x 0.2ml -
Research areas
Images
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Carbohydrates in food items and mouse tissue lysates, shown as mg per mg of extracted protein (duplicates; +/- SD).
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Carbohydrates measured in orange juice and caster sugar. Background signal subtracted, duplicates; +/- SD.
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D-Glucose standard curve generated using the method described in the protocol bookelt.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab155891 has been referenced in 6 publications.
- Kortright KE et al. Selection for Phage Resistance Reduces Virulence of Shigella flexneri. Appl Environ Microbiol 88:e0151421 (2022). PubMed: 34788068
- Martínez DP et al. PAMPs of Piscirickettsia salmonis Trigger the Transcription of Genes Involved in Nutritional Immunity in a Salmon Macrophage-Like Cell Line. Front Immunol 13:849752 (2022). PubMed: 35493529
- Rawashdeh RY et al. Biological interaction levels of zinc oxide nanoparticles; lettuce seeds as case study. Heliyon 6:e03983 (2020). PubMed: 32509982
- Greco CM et al. A non-pharmacological therapeutic approach in the gut triggers distal metabolic rewiring capable of ameliorating diet-induced dysfunctions encompassed by metabolic syndrome. Sci Rep 10:12915 (2020). PubMed: 32737396
- Terashima M et al. Antarctic heterotrophic bacterium Hymenobacter nivis P3T displays light-enhanced growth and expresses putative photoactive proteins. Environ Microbiol Rep 11:227-235 (2019). PubMed: 30298689
- Ear PH et al. Maternal Nicotinamide Riboside Enhances Postpartum Weight Loss, Juvenile Offspring Development, and Neurogenesis of Adult Offspring. Cell Rep 26:969-983.e4 (2019). PubMed: 30673618