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Total Bile Acids (TBA) Assay Kit (Colorimetric)

A simple, sensitive & easy colorimetric assay to quantitate total bile acids.
Catalog #: K209

Product Details

Cat # +Size K209-100
Size 100 assays
Detection Method Absorbance (405 nm).
Species Reactivity Mammalian
Applications Estimation of Bile Acids in various biological samples.
Features & Benefits • Simple, fast and convenient assay
• Can detect as low as 1 µM Bile acids in a variety of samples.
Kit Components • TBA Cycling Assay Buffer
• TBA Probe Buffer
• TBA Probe
• TBA Cycling Enzyme Mix
• TBA Standard (100 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Bile Acids (BA) make 67% of the total composition of Bile. They are 24-carbon steroids generated during cholesterol metabolism. They form conjugates with either glycine or taurine to form bile salts. Five of the BAs account for more than 99% of the total population found in biofluids. The average composition in healthy individuals includes conjugates of cholic, chenodeoxycholic, deoxycholic and lithocholic acids. Bile acids are critical due to their ability to solubilize lipids by forming micelles with cholesterol, and fatty acids. Their synthesis is not only critical for the removal of cholesterol from the body abut they are also needed for proper uptake of dietary lipids into the small intestine. The measurement of circulatory Total Bile Acids (TBA) therefore provides information about hepatic functions and liver diseases such as jaundice, and hepatocellular injury. TBA estimation can detect liver damage during early stages and permits patients to get treatment before hepatic damages become irreversible. In addition, Bile Acids participate as signaling molecules interacting with G-protein coupled receptors (GPCR), TGR5, and nuclear receptor farnesoid X receptor (FXR). BioVision’s Total Bile Acid Assay Kit provides a simple, sensitive, and high-throughput adaptable approach to detect physiological concentration of total bile acids in a variety of biological fluids. The principle of the assay is based on an enzymatic cycling method in the presence of NADH and a chromophore. The reduction of the chromophore produces a stable colorimetric product the absorbance of which can be followed kinetically at 405 nm. This absorbance is directly proportional to the amount of TBA in the sample. Our assay is very specific and sensitive. Other metabolites found in biofluids do not interfere with the assay. The assay can detect as little as 1 µM of Bile Acids in a variety of samples.

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Can the kit distinguish between glycolic and taurocholic acid?
The kit can detect any of the bile acids- however it cannot distinguish between them. In other words- it can definitely measure the Total bile acid in a sample but it cannot specifically say if its glycolic or taurocholic.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian samples only.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Zhou Zhou, Adipose‐Specific Lipin‐1 Overexpression Renders Hepatic Ferroptosis and Exacerbates Alcoholic Steatohepatitis in Mice. Hepatol Commun, May 2019; 31061954.
For more citations of this product click here