Product Details
Cat # +Size | K274-100 |
---|---|
Size | 100 assays |
Detection Method | Absorbance (570 nm) |
Species Reactivity | Mammalian |
Applications | Assay measuring either the combination of both small molecule and protein antioxidants or small molecules antioxidant alone in the presence of our proprietary Protein Mask. |
Features & Benefits | • Simple procedure; takes less than 2 hours • Fast and convenient |
Kit Components | • Cu²ᶧ Reagent • Assay Diluent • Protein Mask • Trolox Standard |
Storage Conditions | 4°C |
Shipping Conditions | Gel Pack |
USAGE | For Research Use Only! Not For Use in Humans. |
Details
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1. Different lots may give different readings. However, when using calibration curve together with the sample testing, the sample concentration should be in the same range.
2. Sample may change during storage, especially Vitamin C.
3. When using the same lot, the same sample reading should be similar, otherwise maybe there was some experimental error.
1) This method quantifies DPPH after exposure to sample-The standard curve gives negative results.
2) Reaction kinetics between DPPH and anti oxidants are not linear. So the anti oxidant capacity is rather arbitrary.
3) The time to steady state using DPPH varies with different anti oxidants. So we may get conflicting relative capacity depending on the reaction time.