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Product Details
| Cat # +Size | E4747-100 |
|---|---|
| Size | 100 assays |
| Detection Method | Absorbance (450 nm) |
| Species Reactivity | Universal |
| Applications | • Sensitivity: 0.02 ppb (ng/ml) • Sample recovery rate: Grain/Formula feed- 85%±15%, Peanut- 82%±15%, Edible oil- 85%±15%, Sauce/Wheat/Barley feed- 83%±15%, Beer- 84%±15%, Wine/Soy sauce/Vinegar- 87%±15% • Detection limit: Grain- 0.1 ppb; Formula feed- 0.2 ppb; Edible oil/Peanut- 0.2 ppb; Sauce/Wheat/Barley feed- 0.2 ppb; beer- 0.2 ppb; Wine/Soy sauce/Vinegar- 0.1 ppb, quantitative measurement of Total Aflatoxin in grain and feed samples. • Cross-reactivity: Aflatoxin B1 (AFB1) - 100%, AflatoxinB2 (AFB2) - 80%, AflatoxinG1 (AFG1)- 75%, AflatoxinG2 (AFG2) - 45%, FlatoxinM1 (AFM1) - 8% |
| Features & Benefits | • Cross-reactivity: Aflatoxin B1 (AFB1) - 100%, AflatoxinB2 (AFB2) - 80%, AflatoxinG1 (AFG1)- 75%, AflatoxinG2 (AFG2) - 45%, FlatoxinM1 (AFM1) - 8% • Quantitative measurement of Total Aflatoxin in grain and feed samples. • Detection limit: Grain- 0.1 ppb; Formula feed- 0.2 ppb; Edible oil/Peanut- 0.2 ppb; Sauce/Wheat/Barley feed- 0.2 ppb; beer- 0.2 ppb; Wine/Soy sauce/Vinegar- 0.1 ppb • Sensitivity: 0.02 ppb (ng/ml) • Sample recovery rate: Grain/Formula feed- 85%±15%, Peanut- 82%±15%, Edible oil- 85%±15%, Sauce/Wheat/Barley feed- 83%±15%, Beer- 84%±15%, Wine/Soy sauce/Vinegar- 87%±15% |
| Kit Components | High Concentrated Standard (100 ppb) ELISA Microplate Stop Solution HRP Conjugate Standards (S1 - S6) Substrate Reagent A Wash Buffer (20x) Substrate Reagent B Plate Sealer Antibody Working Solution |
| Storage Conditions | 4°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
This Total Aflatoxin ELISA Kit is based on the Competitive ELISA method. It can detect Aflatoxin (AF) in samples, such as grain, peanut, formula feed, etc. The microplate provided in this kit has been pre-coated with coupled antigen. During the reaction, AF in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-AF antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of AF. The concentration of AF in the samples can be calculated by comparing the OD of the samples to the standard curve.
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