TNF-alpha, murine recombinant

A potent cytokine exerting cytotoxic effects on a wide range of cells

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Catalog #: 1051 | abID:

Product Details

Alternate Name Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2
Gene Symbol TNFA
Gene ID 21926
Accession # P06804.2
Source E. coli.
Appearance Lyophilized powder
Physical Form Description Steriled and lyophilized from 5 mM Tris, pH 8.0.
Molecular Weight 17.5 kDa
Purity by SDS-PAGE ≥98%
Purity by ≥98%
Endotoxin Level <0.1 ng/μg
Biological Activity The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is < 1 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg.
Reconstitution Instructions Reconstitute in H₂O to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

Tumor Necrosis Factor-α TNF-α) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Murine TNF-α is a 17.5 kDa protein containing 156 amino acid residues.


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Srivastava SK et al. (2007) J. Biol. Chem. 282: 282: 35113 - 35124
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