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TMRE Mitochondrial Membrane Potential Assay Kit (Fluorometric)

based on 1 citations in multiple journalsTMRE Mitochondrial Membrane Potential Assay Kit (Fluorometric)14.1 4
Simple assay to measure mitochondrial membrane potential
Catalog #: K238

Product Details

Cat # +Size K238-200
Size 200 assays
Detection Method Fluorescence (Ex/Em = 535/587 nm), FACS [FL-3 (Red) channel], Fluorescent microscopy [red filter (Ex/Em = 549/575 nm)]
Applications Labeling of active mitochondria in cells
Screening of compounds which may compromise mitochondrial membrane potential
Features & Benefits • Convenient method
• Based on cell-permeable, positively-charged, red-orange dye ™RE, that readily accumulates in the active mitochondria
• Includes Protonophores FCCP to validate the assay
Kit Components • Assay Buffer
• ™RE Dye (1 mM, in DMSO)
• Negative Control (FCCP, 40 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Mitochondria are the powerhouse of the cells and generate energy in the form of ATP. Mitochondria utilize oxidizable substrates to produce a membrane potential in the form of a proton gradient across the mitochondrial inner membrane. Mitochondrial membrane potential is tightly interlinked to many mitochondrial processes, including ATP synthesis, ROS, calcium sequestration and import of proteins into the mitochondrion. Therefore, pharmacological changes in mitochondrial membrane potential are associated with several pathological parameters. BioVision’s ™RE Mitochondrial Membrane Potential Assay Kit uses ™RE (tetramethylrhodamine, ethyl ester) to label active mitochondria. ™RE is a cell-permeable, positively-charged, red-orange dye that readily accumulates in active mitochondria. Protonophores like FCCP and CCCP induces dissipation of mitochondrial membrane thus results in compromised staining.

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Wu J et al., (2017) Redox imbalance and mitochondrial abnormalities in the diabetic lung, Redox Biol, 2017, 11:51-59
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