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TMB, Ultrasensitive

A peroxidase substrate

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to
Catalog #: 1215

Product Details

Cat # +Size 1215-100
Size 100 ml
Alternate Name 3,3',5,5'-Tetramethylbenzidine; TMBUS
Appearance Liquid
Formulation 2.08 mMol L⁻¹ and Hydrogen Peroxide, citric acid buffer at pH 3.3.
CAS # 54827-17-7
Molecular Formula C₁₆H₂₀N₂
Molecular Weight 240.35
InChi InChI=1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
PubChem CID 41206
MDL Number MFCD00007748
Handling Protect from light
Storage Conditions 4°C
Shipping Conditions RT
USAGE For Research Use Only! Not For Use in Humans.


3,3'5,5'-Tetramethylbenzidine (TMB) has been shown to be a safe-sensitive substrate for the assay of horseradish peroxidase (HRP). Initially, in the presence of HRP and hydrogen peroxide, a one-electron oxidation product is formed. This compound, a cation free radical, is blue in color with an adsorption maximum at 653 nm. Further reaction with HRP/H₂O₂ or acidification of the radical with acid yields the diimine terminal oxidation product adsorbing light at 450 nm. The extinction coefficient of the radical (E653 nm = 3.9 x 10⁴ mo⁻¹1 cm⁻¹) and diimine (E450 nm = 5.9 x 10⁴ mol⁻¹ cm⁻¹) provide a remarkably sensitive system for the assay of HRP and HRP labeled probes. TMBUS, available from Biovision Inc., is a single component reagent stable at room temperature and not sensitive to normal laboratory light. It is optimized with respect to TMB and hydrogen peroxide concentrations and yields a linear response with the concentrations of HRP usually employed in immunologic assays.

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