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Tissue Plasminogen Activator (tPA) Activity Assay Kit (Colorimetric)

The most sensitive assay to measure tPA activity in biological samples
Catalog #: K178

Product Details

Cat # +Size K178-100
Size 100 assays
Kit Summary tPA Activity Assay Kit (Fluorometric): Quantitative 96-well plate format assay of tPA activity in biological samples. Simple & Sensitive; Includes active tPA as positive control. 100 reactions.
Detection Method Colorimetric (OD: 405 nm)
Species Reactivity All
Applications Analysis and study of Fibrinolytic system.,Measurement of tPA activity in various Biological Fluids, Tissues and Cell Culture Supernatants
Features & Benefits • Non-Radioactive
• Highthroughput compatible
• Rapid easy one-step assay
• Rapid assessment of enzymatic activity of tPA in biological samples
Kit Components • tPA Positive Control
• Inhibitor Mix (10 mM)
• tPA Substrate
• tPA Assay Buffer
• pNA Standard (0.1 M)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Tissue plasminogen Activator (tPA, PLAT or tissue-type plasminogen activator; EC is a serine protease that is found in blood & some tissues. tPA is the major intravascular activator of plasminogen. Thus, tPA is a key enzyme and the primary initiator of fibrinolysis in the vascular system where it plays an important role in the breakdown of blood clots. tPA has therapeutic uses and it is known as the “clot buster” in clinical settings. High tPA activity in blood leads to hyperfibrinolysis and causes excessive bleeding; while low tPA activity leads to hypofibrinolysis, a condition that increases patient’s risks of suffering an arterial thrombosis. BioVision’s Tissue Plasminogen Activator (tPA) Activity Assay Kit utilizes the ability of active tPA to hydrolyze the synthetic substrate releasing pNA (chromophore). The released pNA can be easily quantified by OD: 405 nm. Our kit uses a unique combination of Substrate and Inhibitor Mix that specifically detects tPA in a variety of Biological Samples. Other enzymes with similar catalytic properties [i.e. uPA (urokinase or urokinase-type plasminogen activator)] do not interfere with the assay. Our assay kit is simple, specific and can detect as low as 5 µU of tPA activity.

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Can I use frozen mouse blood as a sample? Or should I always be using freshly collected blood?
You can use frozen plasma and not whole blood for the assay. Isolate plasma from blood and treat it with 0.1 M citrate before its frozen. We do not recommend using whole blood for the assay as it seems to have a lot more interference as compared to plasma samples.