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TIMP-2 (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of human TIMP-2 in serum, plasma and other biological fluids.
Catalog #: E4841
$525.00

Product Details

Cat # +Size E4841-100
Size 96 assays
Detection Method Sandwich ELISA, Absorbance (450 nm)
Species Reactivity Human
Applications • in-vitro quantitative determination of Human TIMP-2 concentrations in serum, plasma and other biological fluids.
Features & Benefits • Specificity: No Significant cross-reactivity or interference between Human TIMP-2 and analogues was observed.
• Precision: Coefficient of variation is < 10%
• Sensitivity: 0.10 ng/mL
• Detection Range: 0.16-10 ng/mL
Kit Components • Micro ELISA Plate
• Reference Standard
• Biotinylated Detection Ab (100x)
• HRP Conjugate (100x)
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Wash Buffer (25X)
• Substrate Reagent
• Stop Solution
• Plate Sealer
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

TIMP metallopeptidase inhibitor 2 is also known as TIMP-2, which belongs to the protease inhibitor I35 (TIMP) family. This family proteins are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, TIMP2 has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. BioVision’s TIMP-2 (Human) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TIMP-2. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Human TIMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of TIMP-2 bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human TIMP-2. The concentration of Human TIMP-2 in the samples can be calculated by comparing the OD of the samples to the standard curve.


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