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Product Details
| Cat # +Size | E4943-100 |
|---|---|
| Size | 96 assays |
| Kit Summary | Tilmicosin is a semi-synthetic macrolide antibiotic that is structurally similar to Tylosin. As a macrolide, its main function is to bind to 23s rRNA of the 50S ribosomal subunit of the bacteria. In doing so, it prevents the transpeptidation/translocation step and thereby inhibits bacterial protein synthesis. It targets both gram-positive and gram-negative bacteria that cause respiratory infection in cattle and pigs. BioVision’s Tilmicosin ELISA kit is used to quantitatively measure Tilmicosin in muscle and liver tissue, honey, milk, and egg samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Tilmicosin antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Tilmicosin in the samples. |
| Detection Method | Absorbance (450 nm) |
| Species Reactivity | Universal |
| Applications | Competitive ELISA kit to quantitatively measure Tilmicosin in tissue, honey, milk, and egg samples |
| Features & Benefits | • This ELISA kit is used for in vitro quantitative determination of Tilmicosin
• 0.5ppb for muscle (Method 1), 10ppb for muscle (Method 2), 10ppb for liver, 1ppb for honey, 5ppb for milk and eggs • Cross reaction: Tilmicosin 100%, Tylosin 1% • Sensitivity: 0.5ppb |
| Kit Components | • Micro ELISA Plate
Plate Sealer • Standard (S0 – S5) • HRP Conjugate • Antibody working solution • Substrate A • Substrate B • Stop Solution • Wash Buffer (20X) • Redissolving Solution (2X) |
| Storage Conditions | 4ºC |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Tilmicosin is a semi-synthetic macrolide antibiotic that is structurally similar to Tylosin. As a macrolide, its main function is to bind to 23s rRNA of the 50S ribosomal subunit of the bacteria. In doing so, it prevents the transpeptidation/translocation step and thereby inhibits bacterial protein synthesis. It targets both gram-positive and gram-negative bacteria that cause respiratory infection in cattle and pigs. BioVision’s Tilmicosin ELISA kit is used to quantitatively measure Tilmicosin in muscle and liver tissue, honey, milk, and egg samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Tilmicosin antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Tilmicosin in the samples.
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