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TGF-beta1 (human) ELISA Kit

Sensitive, Colorimetric Assay
Catalog #: K4342
$635.00

Product Details

Cat # +Size K4342-100
Size 100 assays
Detection Method Absorbance (450 nm)
Species Reactivity human
Applications This ELISA kit is used for quantitative protein detection, establishing normal range.
Features & Benefits • Easy, convenient and reliable method to assay for human TGF-beta 1.
• Detection Range: 15.6-1000 pg/ml. Sensitivity: < 1 pg/ml.
• This ELISA kit shows <1% cross-reactivity with TGF?2, TGF?3 & TGF?5.
• Cross Reactivity: No significant cross-reactivity or interference between this analyte and its analogues was observed.
Kit Components • 96 wells coated with anti-human TGF-β1 antibody, 1 Microplate
• Lyophilized recombinant human TGF-β1 (25KDa) standard (10 ng/vial)
• Biotinylated anti-human TGF-β1 antibody
• Avidin-Biotin-Peroxidase Complex (ABC)
• Sample diluent buffer
• Antibody diluent buffer
• ABC diluent buffer
• TMB color developing agent (Colorless)
• TMB stop solution
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

TGF-β1 is a multifunctional cytokine involved in cell growth, differentiation and inflammatory pathways. BioVision’s human TGF-β1 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for human TGF-β1 is coated on a 96-well plate. Standards and test samples are added to the wells and TGF-β1 present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for TGF-β1 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human TGF-β1 captured onto the plate. This ELISA kit shows <1% cross-reactivity with TGF-β2, TGF-β3 and TGF-β5. Detection Range: 15.6 pg/ml – 1000 pg/ml. Sensitivity: < 1 pg/ml.


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