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TFEB Transcription Factor Activity Assay Kit (Colorimetric)

A 96-well plate based colorimetric assay to measure the activation of TFEB in nuclear extracts or cell lysates

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: K2088
$695.00

Product Details

Cat # +Size K2088-100
Size 100 assays
Kit Summary BioVision’s TFEB Transcription Factor Activity Assay is a 96-well plate based colorimetric assay to measure the activation of TFEB in nuclear extracts or cell lysates.
Detection Method Absorbance at 450 nm
Applications Semi-quantitative measurement of activation of human TFEB in nuclear extracts or cell lysates.
Features & Benefits The kit offers a easy, rapid, sensitive and non-radioactive way to detect the activation of TFEB in samples.
Kit Components ● Plate Coated with DNA Probes
● Non-Competitor Oligo (20 pmole)
● TMB Substrate
● Stop Solution
● Positive Control
● Plate Sealing Film
● Binding Buffer (5X)
● DTT (100 mM)
● Protease Inhibitor Cocktail
● TFEB Primary Antibody
● Antibody Diluent Buffer
● HRP Conjugate Stock
● Wash Buffer (10X)
● Competitor Oligo (20 pmole)
Storage Conditions Multiple Temperatures
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not For Use in Humans.

Details

The transcription factor EB (TFEB) gene encodes a transcription factor, which is a master regulator of lysosomal biogenesis and exocytosis in autophagy. In normal state, TFEB gets phosphorylated via mTOR and MAPK pathways and is present in the cytoplasm. However, upon starvation or lysosomal stress, TFEB gets dephosphorylated and is translocated to the nucleus where it binds to the CLEAR consensus DNA sequence (5’-GTCACGTGAC-3’) in the regulatory region of the lysosomal genes and induces the transcription of the downstream genes. Traditionally, western blot is used to detect the expression of TFEB and electrophoretic mobility shift assay (EMSA) or reporter assays are used to measure the TFEB activity respectively. But some of these methods are time consuming, laborious, and uses radioactivity. BioVision’s TFEB Transcription Factor Activity Assay is a 96-well plate based colorimetric assay to measure the activation of TFEB in nuclear extracts or cell lysates. The kit offers a easy, rapid, sensitive and non-radioactive way to detect the activation of human TFEB in samples. In this assay, double stranded oligonucleotides are coated on the 96-well plate. The cell lysate or the nuclear extract containing the active TFEB is then added to the wells, which binds to the oligonucleotides on the plate. After the addition of TFEB primary antibody that recognizes the TFEB-oligonucleotide complex, a HRP-conjugated secondary antibody is added followed by the addition of TMB substrate and a color signal is developed, which is measured at 450 nm.


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