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TEV Protease Inhibitor Screening Kit (Fluorometric)

Markets's first and only HTP adaptable assay kit to screen/study/characterize potential inhibitors of TEV Protease
Catalog #: K843
$325.00

Product Details

Cat # +Size K843-100
Size 100 assays
Detection Method Fluorescence (Ex/Em = 490/560 nm)
Applications Screening/studying/characterizing inhibitors of TEV Protease.
Features & Benefits Simple, rapid assay
HTP Adaptable
Fluorometric Detection
Kit Components • TEV Protease Assay Buffer
• TEV Protease
• TEV Protease Substrate
• TEV Protease Inhibitor (2 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

TEV Protease (EC: 3.4.22.44) is a cysteine protease that recognizes the cleavage site of Glu-Xaa- Xaa-Y- Xaa-Gln-(Gly/Ser) and cleaves between Gln and Gly/Ser. The optimal sequence is Glu-Asn-Leu-Tyr-Phe-Gln-Ser/Glycine (ENLYFQS/G). TEV Protease has high specificity and great stability and is active over a wide range of temperatures (4-37°C) with an optimal activity at 34°C. BioVision’s Inhibitor Screening Kit utilizes the ability of TEV Protease to cleave a synthetic Fluorescein-based peptide substrate to release fluorescein which can be easily quantified using a fluorometer or fluorescence microplate reader (Ex/Em = 490/560 nm). In the presence of a TEV Protease-specific inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of TEV Protease.


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