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TEV Protease Activity Assay Kit (Fluorometric)

Simple kit with a fast protocol to detect as low as 50 ng/well of TEV Protease activity in viral lysates and purified protein samples.
Catalog #: K842
$415.00

Product Details

Cat # +Size K842-100
Size 100 assays
Detection Method Fluorescence (Ex/Em = 490/560 nm)
Species Reactivity Viral lysates
Applications Detection of TEV Protease activity in viral lysates
Determine activity of purified TEV Protease
Features & Benefits Simple, rapid assay
Can detect as low as 50 ng/well of TEV Protease activity
Fluorometric Detection
Kit Components • TEV Protease Assay Buffer
• TEV Protease Dilution Buffer
• 1 M DTT
• TEV Protease (Positive Control)
• TEV Protease Substrate
• 5-FAM Standard (100 µM in DMSO)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

TEV Protease (EC: 3.4.22.44, Tobacco Etch Virus protease) is a cysteine protease that recognizes the cleavage site of Glu-Xaa- Xaa-Y- Xaa-Gln-(Gly/Ser) and cleaves between Gln and Gly/Ser. The optimal sequence is Glu-Asn-Leu-Tyr-Phe-Gln-Ser/Glycine (ENLYFQS/G). TEV Protease has high specificity and great stability and is active over a wide range of temperatures (4-37°C) with an optimal activity at 34°C. BioVision’s TEV Protease Activity assay kit utilizes the ability of TEV Protease to cleave a synthetic Fluorescein-based peptide substrate to release fluorescein which can be easily quantified using a fluorometer or fluorescence microplate reader (Ex/Em = 490/560 nm). This assay kit is simple, rapid and can detect TEV Protease activity as low as 50 ng/well in viral lysates and purified protein samples.


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