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T4 DNA ligase

Joins blunt-end or stick-end DNA fragments
Catalog #: M1514

Product Details

Cat # +Size M1514-4000
Size 4000 U
Highlights ● For blunt-end and cohesive-end DNA ligation
● Cloning restriction fragments
● Ligate linkers and adapters to blunt-ended DNA
● RNase, nuclease and exonuclease free
● No residual host genomic DNA detected by PCR
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme joins blunt-end and cohesive-end termini as well as repairs single-stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA Ligase seals nicks for these DNA substrates. T4 DNA Ligase can be used for cloning restriction fragments and joining linkers and adapters to blunt-ended DNA.

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