Anti-Syndecan 4 antibody (ab286154)
Key features and details
- Rabbit polyclonal to Syndecan 4
- Suitable for: WB, IHC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Syndecan 4 antibody
See all Syndecan 4 primary antibodies -
Description
Rabbit polyclonal to Syndecan 4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHCmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human Syndecan 4. Synthetic peptide surrounding amino acid 184 of human Syndecan-4
Database link: P31431 -
Positive control
- WB: Jurkat cell lysate. IHC-P: Human colon tissue.
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General notes
This product is manufactured by BioVision, an Abcam company and was previously called 3644 Syndecan-4 Antibody. 3644-100 is the same size as the 100 µg size of ab286154.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 30% Glycerol (glycerin, glycerine), 0.5% BSA, 69% PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab286154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 22 kDa.
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IHC |
Use a concentration of 5 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 22 kDa. |
IHC
Use a concentration of 5 µg/ml. |
Target
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Function
Cell surface proteoglycan that bears heparan sulfate. -
Tissue specificity
Expressed in epithelial and fibroblastic cells. -
Sequence similarities
Belongs to the syndecan proteoglycan family. -
Post-translational
modificationsShedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3. -
Cellular localization
Membrane. Secreted. Shedding of the ectodomain produces a soluble form. - Information by UniProt
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Database links
- Entrez Gene: 6385 Human
- Entrez Gene: 20971 Mouse
- Entrez Gene: 24771 Rat
- SwissProt: P31431 Human
- SwissProt: O35988 Mouse
- SwissProt: P34901 Rat
- Unigene: 632267 Human
- Unigene: 3815 Mouse
see all -
Alternative names
- Amphiglycan antibody
- MGC22217 antibody
- OTTHUMP00000031788 antibody
see all
Images
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Anti-Syndecan 4 antibody (ab286154) at 1/1000 dilution + Jurkat cell lysate at 100 µg
Secondary
Goat Anti-Rabbit IgG peroxidase conjugated at 1/10000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa100 µg Jurkat cell lysate was loaded. Blocking/Dilution buffer: 5% NFDM (1 hr at RT) / TBST. ab286154 was used at 1/1000 dilution), O/N at 4 °C. Goat Anti-Rabbit IgG peroxidase conjugated used at 1/10,000 dilution, 1 hr at RT.
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Immunohistochemistry of formalin fixed, paraffin-embedded human colon tissue after heat-induced antigen retrieval. 5 µg/ml of antibody was used for staining. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab286154 has been referenced in 1 publication.
- Zhu Z et al. Syndecan-4 is the key proteoglycan involved in mediating sepsis-associated lung injury. Heliyon 9:e18600 (2023). PubMed: 37576224