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Surfactant Protein-D (SP-D) (Human) ELISA Kit

A Sandwich ELISA kit to quantitatively measure SP-D in human serum, plasma, tissue lysates, and other biological fluids
Catalog #: E4896

Product Details

Cat # +Size E4896-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This Sandwich ELISA kit is designed to quantitatively measure amount of SP-D in human serum, plasma, tissue lysates, and other biological fluids
Features & Benefits • Recovery range: 85 - 105% for normal human serum and plasma samples
• Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
• Sensitivity: 0.938 ng/ml
• This Sandwich ELISA is highly sensitive and highly specific for the detection of SP-D in human samples. There is no significant cross-reactivity or interference between SP-D and analogues
• Detection range: 1.563 - 100 ng/ml
Kit Components • Micro ELISA plate
• Standard (Lyophilized) (100 ng)
• Sample/Standard Dilution Buffer
• Biotin-labeled Antibody
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate Solution
• Stop Solution
• Wash Buffer (25X)
• Plate Sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Surfactant protein D (SP-D) is a type of lectin that belongs to the family of collectins, which are a group of collagen-containing C-type lectins. It is produced and secreted by type II alveolar cells, bronchiolar epithelial cells, and Clara cells. SP-D acts as a pattern recognition receptor; it binds to several pathogens of bacterial, fungal, and viral origin and promotes their clearance via phagocytosis. Additionally, SP-D is known to aggregate allergens and assist in their removal by stimulating an allergenic response. SP-D also binds to inflammatory ligands and inhibits pulmonary inflammation. Due to its role in phagocytosis and anti-inflammation, SP-D could serve as an efficient target for the treatment of pulmonary diseases such as chronic obstructive pulmonary disease, asthma, acute interstitial pneumonia (ARDS), and bronchopulmonary dysplasia. BioVision’s SP-D (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the concentration of SP-D in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the concentration of SP-D in the sample or standard.

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