Sulfo-NHS-LC-Biotin is a water-soluble protein biotinylation reagent.
Catalog #: 2326 | abID: ab145611

Product Details

abID ab145611
Alternate Name Sulfosuccinimidyl 6-(biotinamido) Hexanoate; Biotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt
Appearance White to Off-white Solid
CAS # 127062-22-0
Molecular Formula C₁₄H₁₈O₈N₃S₂Na
Molecular Weight 556.59
Purity ≥90% by NMR
Solubility H₂O (~ 20 mg/ml)
InChi InChI=1S/C20H30N4O9S2.Na/c25-15(7-4-3-6-13-18-12(11-34-13)22-20(29)23-18)21-9-5-1-2-8-17(27)33-24-16(26)10-14(19(24)28)35(30,31)32;/h12-14,18H,1-11H2,(H,21,25)(H2,22,23,29)(H,30,31,32);
PubChem CID 16219001
MDL Number MFCD00078534
Handling Protect from moisture
Storage Conditions -20 ̊C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


KEY FEATURES:• Amine-reactive; has a spacer with arm length of 22.4 Å which helps to minimize steric hindrance to biotin labeling• More water-soluble than the corresponding NHS compound without the sulfo group• Membrane-impermeable• Forms permanent amide bonds and the spacer arm cannot be cleavedAPPLICATIONS:• Sulfo-NHS-LC-Biotin is ideal for biotinylating antibody or protein that can then be used for protein detection, purification or immobilization • Sulfo-NHS-LC-Biotin is particularly useful for targeting cell surface proteins due to the membrane-impermeability of this reagent

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When a protein is biotinylated by Sulfo-NHS-LC-Biotin, which part of this molecule attached to protein?
Sulfo-NHS-LC-biotin (Sulfosuccinimidyl 6-(biotinamido) Hexanoate) is an amine-reactive biotinylation agent. It covalently binds to the amine groups in proteins and other molecules through its NHS ester forming an amide linkage.