Sulfo-LC-SPDP (ab285422)
Key features and details
- amine and thiol crosslinking reagent
- CAS Number: 169751-10-4
- Purity: >= 90%
Soluble in water to 10 mM
- Form / State: Solid
Overview
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Product name
Sulfo-LC-SPDP -
Description
amine and thiol crosslinking reagent -
Alternative names
- Sodium salt
- Sulfo-LC-SPDP Crosslinker
- Sulfo-SPDP-C6-NHS (sodium)
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Biological description
Sulfo-LC-SPDP releases a byproduct, pyridine-2-thione after reacting with free sulfhydryl groups. By measuring the release of this byproduct at 343 nm, the reaction can be easily followed.
Amine-reactive; the "long chain" Sulfo-LC-SPDP crosslinking reagent has a 15.7 A° spacer arm.
More water-soluble than the corresponding LC-SPDP.
Membrane-impermeable which enables crosslinking on cell surface.
The cleavable spacer arm enables separation of cross-linked products.
APPLICATIONS:
Reversible crosslinking of proteins.
N-hydroxysulfosuccinimide (Sulfo-NHS) ester that reacts with primary amines, as found in lysine side chains.
Sulfo-LC-SPDP crosslinking molecule pyridinyldisulfide reacts with sulhydryls to yield a reversible disulfide bond.
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Purity
>= 90% -
General notes
This product is manufactured by BioVision, an Abcam company and was previously called 2325 Sulfo-LC-SPDP. 2325-50 is the same size as the 50 mg size of ab285422.
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CAS Number
169751-10-4
Properties
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Chemical name
sodium;2,5-dioxo-1-[6-[3-(pyridin-2-yldisulfanyl)propanoylamino]hexanoyloxy]pyrrolidine-3-sulfonate
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Molecular weight
527.57 -
Molecular formula
C18H22N3NaO8S3 -
PubChem identifier
4588467 -
Storage instructions
Shipped at 4°C. Store at -20°C. Store under desiccating conditions. -
Solubility overview
Soluble in water to 10 mM
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Handling
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
C1C(C(=O)N(C1=O)OC(=O)CCCCCNC(=O)CCSSC2=CC=CC=N2)S(=O)(=O)[O-].[Na+]
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab285422 has not yet been referenced specifically in any publications.