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Sulfamonomethoxine ELISA Kit

Catalog #: E4774
$525.00

Product Details

Cat # +Size E4774-100
Size 96 assays
Detection Method Absorbance (450 nm)
Applications in vitro quantitative determination of Sulfamonomethoxine concentrations in Tissue, Serum, Urine, Honey, Milk samples
Features & Benefits • Cross-reactivity: Sulfadiazine (SD or SDZ) 40%, Sulfamerazine(SM1) 25%, Sulfametoxydiazine(SMD) 50%, Sulfadoxine (SDM') 35%
• Detection limit: Tissue (high detection limit) - 0.5 ppb, Tissue (low detection limit) - 0.25 ppb, Serum/urine - 0.2 ppb, Honey- 0.05 ppb, Milk- 1 ppb.
• Sensitivity: 0.05 ppb (ng/mL)
Kit Components • HRP Conjugate
• Wash Buffer (2X)
• Plate Sealer
• Substrate Reagent A
• Standard
• Stop Solution
• Reconstitution Buffer
• ELISA Microplate
• Antibody Working Solution
• Substrate Reagent B
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Sulfamonomethoxine is a long-acting sulfonamide antibacterial agent, used in blood kinetic studies, and blocks the synthesis of folic acid by inhibiting synthetase of dihydropteroate. BioVision Sulfamonomethoxine ELISA Kit is based on the Competitive ELISA principle. It can detect Sulfamonomethoxine in tissue, serum, honey, milk, urine samples. The micro-plate provided in this kit has been pre-coated with Sulfamonomethoxine. During the reaction, Sulfamonomethoxine in the samples or standard competes with Sulfamonomethoxine coated on the plate for binding to the anti-Sulfamonomethoxine antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microplate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Sulfamonomethoxine. The concentration of Sulfamonomethoxine in the samples can be calculated by comparing the OD of the samples to the standard curve.


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