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Sulfametoxydiazine ELISA Kit

A Competitive ELISA kit for in vitro quantitative determination of Sulfametoxydiazine concentrations in Tissue, Serum, Urine, Honey, Milk samples
Catalog #: E4775

Product Details

Cat # +Size E4775-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications in vitro quantification of Sulfametoxydiazine in Tissue, Serum, Urine, Honey, Milk, Feed samples.
Features & Benefits • Detection limit: Tissue (high detection limit) - 0.5 ppb, Tissue (low detection limit) - 0.25 ppb, Serum/urine - 0.2 ppb, Honey - 0.05 ppb, Milk - 1 ppb
• Cross-reactivity: Sulfadiazine (SD or SDZ) - 40%, Sulfamerazine (SM1) - 25%, Sulfadoxine (SDM) - 35%
• Sensitivity: 0.05 ppb (ng/mL)
• Sample recovery rate: Tissue/honey - 95%±25%, Urine/milk/serum - 85%±25%
Kit Components • Plate Sealer
• Reconstitution Buffer
• Antibody Working Solution
• Stop Solution
• Wash Buffer (20X)
• Substrate Reagent A
• Substrate Reagent B
• Standard
• ELISA Microplate
• HRP Conjugate
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Sulfametoxydiazine is a long-acting sulfonamide antibacterial. It is used as a leprostatic agent and in the treatment of urinary tract infections. BioVision Sulfametoxydiazine ELISA Kit is based on the Competitive ELISA principle. It can detect Sulfametoxydiazine in tissue, serum, honey, milk, urine samples. The micro-plate provided in this kit has been pre-coated with Sulfametoxydiazine. During the reaction, Sulfametoxydiazine in the samples or standard competes with Sulfametoxydiazine coated on the plate for binding to the anti-Sulfametoxydiazine antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Sulfametoxydiazine. The concentration of Sulfametoxydiazine in the samples can be calculated by comparing the OD of the samples to the standard curve.

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