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Sulfamethoxazole ELISA Kit

A Competitive ELISA kit for in-vitro quantification of Sulfamethoxazole in food samples

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to
Catalog #: E4770

Product Details

Cat # +Size E4770-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications in-vitro quantification of Sulfamethoxazole in Tissue, Serum, Urine, Egg, Honey, Milk, Feed samples
Features & Benefits • Detection limit: Tissue (high detection limit method) - 0.1 ppb, Tissue (low detection limit method) - 1 ppb, Honey- 0.1 ppb, Serum, Urine, Egg- 0.4 ppb, Milk- 2 ppb, Feed- 4 ppb
• Sensitivity: 0.1 ppb (ng/mL)
• Sample recovery rate: Tissue/Honey/Egg - 85%±25%, Serum/Urine/Milk/Feed- 80%±25%
Kit Components • HRP Conjugate
• Stop Solution
• Plate Sealer
• Reconstitution Buffer (2X)
• Wash Buffer (20X)
• Antibody Working Solution
• Standard
• Substrate Reagent B
• ELISA Microplate
• Substrate Reagent A
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Sulfamethoxazole is an antibiotic that blocks the synthesis of dihydrofolic acid by inhibiting the enzyme dihydropteroate synthase. It inhibits folic acid synthesis in prokaryotes. BioVision’s Sulfamethoxazole ELISA Kit is based on the Competitive ELISA method. It can detect Sulfamethoxazole in samples, such as Tissue, Serum, Urine, Egg, Honey, Milk, Feed. The microtiter plate provided in the kit has been pre-coated with Sulfamethoxazole. During the reaction, Sulfamethoxazole in the samples or standard competes with coated Sulfamethoxazole on the solid phase supporter for sites of anti- Sulfamethoxazole antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of Sulfamethoxazole. The concentration of Sulfamethoxazole in the samples can be calculated by comparing the OD of the samples to the standard curve

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