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Sulfamethazine ELISA Kit

A Competitive ELISA kit for in vitro quantitative determination of Sulfamethazine concentrations in Tissue, Serum, Honey, Milk, Egg, Urine samples

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to
Catalog #: E4778

Product Details

Cat # +Size E4778-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications in vitro quantitative determination of Sulfamethazine concentrations in Tissue, Serum, Honey, Milk, Egg, Urine samples
Features & Benefits • Sensitivity: 0.5 ppb (ng/mL)
• Cross-reactivity: Sulfamethazine - 100%.
• Detection limit: Tissue (high detection limit method) - 0.5 ppb, Tissue (low detection limit method) - 2.5 ppb, Serum, Urine - 2 ppb, Honey- 0.5 ppb, Milk -10 ppb
• Sample recovery rate: Tissue, Honey- 95%±25%, Urine, Milk, Serum- 85%±25%.
Kit Components • Standard
• Stop Solution
• ELISA plate
• Substrate Reagent B
• HRP Conjugate
• Plate Sealer
• Reconstitution Buffer
• Wash Buffer (20X)
• Antibody Working Solution
• Substrate Reagent A
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Sulfamethazine is a sulfonamide consisting of pyrimidine with methyl substituents at the 4- and 6-positions and a 4-aminobenzenesulfonamido group at the 2-position. It has a role as an anti-infective agent, a carcinogenic agent, a ligand, an antibacterial drug, an antimicrobial agent. Sulfamethazine ELISA Kit is based on the Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with Sulfamethazine. During the reaction, Sulfamethazine in the samples or standard competes with Sulfamethazine coated on the plate for binding to the anti-Sulfamethazine antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Sulfamethazine. The concentration of Sulfamethazine in the samples can be calculated by comparing the OD of the samples to the standard curve.

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