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Sucrose Phosphorylase Activity Assay Kit (Colorimetric)

To determine the activity of Sucrose Phosphorylase
Catalog #: K2034
$495.00

Product Details

Size 100 assays
Detection Method Absorbance (OD 450 nm)
Species Reactivity Bacteria
Applications • Analysis of Sucrose Phosphorylase kinetics and inhibition
• Measurement of Sucrose Phosphorylase activity in bacterial lysates
Features & Benefits • Sensitive
• A convenient tool for measuring Sucrose Phosphorylase activity
• It can detect as low as 10 mU under the assay conditions
• Rapid
Kit Components • Sucrose Assay Buffer
• Sucrose
• Sucrose Enzyme Mix
• Sucrose Substrate Mix
• Sucrose Developer
• G1P Standard
• Sucrose Phosphorylase
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Sucrose Phosphorylase (EC 2.4.1.7) is a transglucosidase enzyme, which plays a key role in the metabolism of sucrose, starch as well as other metabolites. It catalyzes the conversion of sucrose to α-D-glucose-1-phosphate (G1P) and D-fructose. It is present in both gram positive and negative bacteria such as in Leuconostoc mesenteroides, Bifidobacterium longum, Pseudomonas sacclwrophila and Lactobacillus reuteri. Sucrose Phosphorylase is used as a biocatalyst to produce fructose syrup in food industry and for determining inorganic phosphate in clinical analysis. BioVision’s Sucrose Phosphorylase Activity Assay Kit can be used to determine the activity of Sucrose Phosphorylase in different microorganisms. The assay converts Sucrose to G1P, which is then detected by a set of enzymatic reactions to generate a colored product. The colored product can be measured by absorbance (OD 450 nm) and is directly proportional to the Sucrose Phosphorylase activity. BioVision’s Sucrose Phosphorylase Activity Assay Kit is a rapid, sensitive and a convenient tool for measuring Sucrose Phosphorylase activity. It can detect as low as 10 mU under the assay conditions.


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