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Sucrose Colorimetric/Fluorometric Assay Kit

No interference from Lactose & Maltose
Catalog #: K626

In stock

$385.00

Product Details

Cat # +Size K626-100
Size 100 assays
Detection Method Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species Reactivity Mammalian
Applications The method can detect 0.0002-10 mM sucrose concentrations.
Features & Benefits • Simple procedure; takes ~1 hour
• Fast and convenient
• Kit contains all necessary reagents for accurate measurement of Sucrose
Kit Components • Sucrose Assay Buffer
• OxiRed™ Probe (in DMSO)
• Invertase (Lyophilized)
• Enzyme Mix (Lyophilized)
• Sucrose Standard (100 nmol/µl)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Sucrose (C₁₂H₂₂O₁₁; FW:342.3), also called table sugar, is one of the most important fuel sources to generate the universal energy molecule ATP. Sucrose is a disaccharide which can be converted into one glucose and one fructose. BioVision’s Sucrose Assay Kit provides a convenient means for specifically measuring sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). Sucrose is converted to glucose and fructose by invertase. The generated glucose is then specifically oxidized to generate a product which reacts with a probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm). Use of proper controls allows for correcting free glucose background. Other disaccharides such as lactose and maltose do not interfere with the assay. The method can detect 0.0002-10 mM sucrose concentrations.


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Will Mannitol be detected by this assay?
Although Mannitol is indirectly related to urine creatinine and is frequently used to prevent renal failure, Mannitol is a sugar alcohol and should not be detected by this assay.
Does a background mix i.e assay buffer, creatinase, enzyme mix, probe have to be performed for the creatinine standards aswell as for samples?
The background control is so that interference from Sarcosine be subtracted from the sample readings. The ackground is not needed for the standards. The zero std well is the background from the reagents in the standards.
Many samples read higher than the highest standard value in the fluorometric assay. 0.4ul of the probe was used but it was not diluted.
The fluorometric assay is at least 10x more sensitive than the colorimetric assay. Is it essential to dilute the probe 10x and then use 0.4ul of it to ensure the readings are not too high.
Is this assay compatible with glucose, acetone, acetate, acetic acid, etc. that typically interfere with Glomerular Filtration Rate measurement?
We have not tested this kit individually in higher than physiological amounts of the moieties you mentioned. In typical samples, to our knowledge sarcosine and creatine can interfere with the assay which is resolved by running appropriate controls. Since the assay does not rely on the physical process of filtration, these moieties are not likely to cause any issues.
Using mouse plasma, 10x higher values than what is reported (by HPLC) was calculated. How can this issue be resolved?
Suggestions for this problem: 1) check the creatine and sarcosine background 2) use serum instead of plasma 3) deproteinize the samples as recommended in the protocol 4) In addition, be aware that there can be an inherent difference in the methods used (HPLC vs. ELISA) which could account for the difference in values after substracting the background. If you check for literature values, I would suggest to look for other ELISA data and see what is reported for this methodology.
Can this kit be used to measure activity of creatine Kinase?
No, this kit is not an enzyme activity assay kit. It only detects Creatinine.
Is deproteinization essential?
Yes, deproteiization is recommended to remove all proteins/enzymes that can create backgound in the assay.
Can individual components of this kit be purchased separately?
Yes, any of the kit's components can be purchased separately without having to buy the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Are trial sizes of this kit available?
Unfortunately, we do not have trial sizes of this kit available. However,for customers based in the US or Canada, a 10% off list price introductory discount can be offered on the list price. For all other customers based out of this area our regional BioVision distributor should be contacted.