Succinate Assay Kit (Colorimetric) (ab204718)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 40 µM
Overview
-
Product name
Succinate Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Quantitative -
Sensitivity
40 µM -
Assay time
0h 30m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Succinate Assay Kit (Colorimetric) (ab204718) is a sensitive, fast and easy-to-use kit. In the succinate assay protocol, Succinate is utilized by Succinyl-CoA Synthetase to form an intermediate, which undergoes a series of reactions and reduces a colorless probe to a colored product with strong absorbance at OD=450 nm. This assay kit can detect less than 40 µM of succinate or succinic acid in a variety of sample types.
Succinate + ATP + CoA converted by succiny-CoA synthase to succiny-CoA + ADP + Pi with Color Detection (OD 450 nm).
Succinate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min
- analyze with a microplate reader -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K649 Succinate (Succinic Acid) Colorimetric Assay Kit. K649-100 is the same size as the 100 test size of ab204718.
Succinic acid (C4H6O4) is distributed in all plants and animal tissues and was first obtained from amber. Due to its low toxicity, it is widely used in agriculture, food and pharmaceutical industry. Succinate (C4H4O4) is the salt or ester of succinic acid. It is one of the most active components of cellular respiratory and intracellular energy generation. Measurement of succinate or succinic acid level is a key to analysis of the citric acid cycle.
This kit has so far only been tested with human and rat samples. Since Succinate is distributed in all plants and animal tissues this kit should detect Succinate independent of the species. Depending on the sample type, the sample preparation might need to be optimized.
-
Platform
Microplate reader
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Developer Solution III 1 vial Enzyme Mix XI 1 vial Assay Buffer VII 1 x 25ml Succinate Converter (Lyophilized) 1 vial Succinate Standard 1 vial Succinate Substrate Mix 1 vial -
Research areas
-
Relevance
Succinic acid is involved in the catabolism of the neurotransmitter gamma-aminobutyric acid (GABA). -
Alternative names
- butanedioic acid
- ethane-12 dicarboxylic acid
- ethylenesuccinic acid
see all
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (36)
ab204718 has been referenced in 36 publications.
- Stifel U et al. Glucocorticoids coordinate macrophage metabolism through the regulation of the tricarboxylic acid cycle. Mol Metab 57:101424 (2022). PubMed: 34954109
- Xiao Y et al. Succinate Is a Natural Suppressor of Antiviral Immune Response by Targeting MAVS. Front Immunol 13:816378 (2022). PubMed: 35309330
- Singh V et al. The Molecular Role of HIF1a Is Elucidated in Chronic Myeloid Leukemia. Front Oncol 12:912942 (2022). PubMed: 35847841
- Patrono D et al. Clinical assessment of liver metabolism during hypothermic oxygenated machine perfusion using microdialysis. Artif Organs 46:281-295 (2022). PubMed: 34516020
- Zhou W et al. SENP1-Sirt3 signaling promotes a-ketoglutarate production during M2 macrophage polarization. Cell Rep 39:110660 (2022). PubMed: 35417703