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Succinate (Succinic Acid) Colorimetric Assay Kit

Only kit on the market to measure succinate (succinic acid)
Catalog #: K649
$720.00

Product Details

Cat # +Size K649-100
Size 100 assays
Detection Method Absorbance (450 nm)
Applications Measurement of succinate or succinic acid in various samples
Analysis of citric acid cycle in various tissues/cells
Features & Benefits • Simple, rapid & convenient
• The assay can detect less than 40 µM of Succinate or Succinic Acid in various sample types.
Kit Components • Succinate Assay Buffer
• Succinate Converter (Lyophilized)
• Succinate Enzyme Mix (Lyophilized)
• Succinate Substrate Mix (Lyophilized)
• Succinate Developer (Lyophilized)
• Succinate Standard (Lyophilized)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Succinic acid (C4H6O4) is distributed in all plants and animal tissues and was first obtained from amber. Due to its low toxicity, it is widely used in agriculture, food and pharmaceutical industry. Succinate (C4H4O4) is the salt or ester of succinic acid. It is one of the most active components of cellular respiratory and intracellular energy generation. Measurement of succinate or succinic acid level is a key to analysis of the citric acid cycle. BioVision’s Succinate (Succinic Acid) Assay kit is a sensitive, fast and easy-to-use kit. In this assay, Succinate is utilized by Succinyl-CoA Synthetase to form an intermediate, which undergoes a series of reactions & reduces a colorless probe to a colored product with strong absorbance at 450 nm. This assay kit can detect less than 40 µM of succinate or succinic acid in a variety of sample types.


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What is the standard in this kit made of? What is its source?
The standard is a lipid and does not have an animal source. The exact details are proprietary and cannot be disclosed.
Can frozen samples be used with this assay?
Fresh samples are always preferred over frozen samples. However, frozen samples can also be used, provided, they were frozen right after isolation, were not freeze thawed multiple time (for which we recommend aliquoting the samples before freezing) and have been frozen for relatively short periods.
Can this kit be used with samples like bacteria, plants, drosophila, yeast etc?
We have optimized the kit with mammalian samples. However, theoretically these kits should work with samples from multiple species/sources. Since the optimal conditions depend on the sample type, the protocol has to be be adapted to fit the samples for efficient results. . Some optimization might be necessary regarding the sample volumes used for best results.
Can flash-frozen tissue be used with this kit?
Yes, tissue flash-frozen in liquid Nitrogen immediately after collection can be used with this assay.
Can a protein assay be used to normalize the loading amount into each well?
Yes, a protein assay can be run with the homogenate/lysate to normalize the loading amount in each well. We suggest using the BCA assay: http://www.biovision.com/bca-protein-assay-kit-ii-6783.html.
Is it essential to make a standard curve for every expt, or is one curve/kit enough?
Yes, it is strongly recommended to do the standards every time you do the expt. There is always a chance that something was done differently that day and we do not want any conditions to differ between standards and samples.