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Succinate Dehydrogenase (SDH) (Rat) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of SDH in rat serum, plasma, culture supernatants and other biological fluids.
Catalog #: E4596

In stock

$695.00

Product Details

Cat # +Size E4596-100
Size 100 assays
Kit Summary • Detection method- Absorbance (450 nm)
• Species reactivity- Rat
• Application- quantitative measurement of Succinate Dehydrogenase in rat serum, plasma, tissue homogenates and other biological fluids.
Detection Method Absorbance (450 nm)
Species Reactivity Rat
Applications This ELISA kit is used for quantitative measurement of Succinate Dehydrogenase in rat serum, plasma, tissue homogenates and other biological fluids.
Features & Benefits • Easy, convenient and time-saving method to measure the level of Succinate Dehydrogenase in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
• Detection Range: 0.313 - 20 ng/ml
• Sensitivity: < 0.188 ng/ml
• Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10% No significant cross-reactivity or interference was observed.
Kit Components • Micro ELISA Plate
• Lyophilized Standard
• Sample / Standard dilution buffer
• Biotin- detection antibody (Concentrated)
• Antibody dilution buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC dilution buffer
• TMB substrate
• Stop Solution
• Wash buffer (25X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Succinate Dehydrogenase (SDH) or succinate-coenzyme Q reductase (SQR) or respiratory complex II is an enzyme complex, which is bound to the inner mitochondrial membrane. SDH participates in both the citric acid cycle and electron transport chain. In mammals and many bacteria, SDH consists of 2 hydrophilic subunits, SDHA (flavoprotein) and SDHB (iron-sulfur protein) and 2 hydrophobic membrane anchor subunits: SDHC and SDHD. SDH oxidizes succinate to fumarate and transfers the electrons to ubiquinone. SDH deficiency in humans leads to a variety of phenotypes including Leigh syndrome, a neurometabolic disorder, tumor formation, and myopathy. Recent studies show that SDH can prevent the generation of ROS (reactive oxygen species); therefore, measurement of succinate dehydrogenase activity has wide applications. BioVision’s Succinate Dehydrogenase ELISA kit is a sandwich ELISA assay for the quantitative measurement of SDH in Rat serum, plasma and cell culture supernatants. The density of color is proportional to the amount of SDH captured from the samples.


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