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Succinate Dehydrogenase Activity Colorimetric Assay Kit

based on 3 citations in multiple journalsSuccinate Dehydrogenase Activity Colorimetric Assay Kit34.1 4
Sensitive assay, HTS
Catalog #: K660
$595.00

Product Details

Cat # +Size K660-100
Size 100 assays
Detection Method Absorbance (OD = 600 nm)
Applications Measurement of Succinate Dehydrogenase Activity in various tissues/cells.
Analysis of citric acid cycle
Features & Benefits • Simple & rapid
• High-throughput adaptable
• This assay kit can detect less than 0.1mU Succinate Dehydrogenase Activity in a variety of samples.
Kit Components • SDH Assay Buffer
• SDH Substrate Mix (Lyophilized)
• SDH Probe
• DCIP Standard (2 mM)
• SDH Positive Control (Lyophilized)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Succinate Dehydrogenase (SDH) (EC 1.3.5.1) or succinate-coenzyme Q reductase (SQR) or respiratory complex II is an enzyme complex, which is bound to the inner mitochondrial membrane. SDH participates in both the citric acid cycle and electron transport chain. In mammals and many bacteria, SDH consists of 2 hydrophilic subunits, SDHA (flavoprotein) and SDHB (iron-sulfur protein) and 2 hydrophobic membrane anchor subunits: SDHC and SDHD. SDH oxidizes succinate to fumarate and transfers the electrons to ubiquinone. SDH deficiency in humans leads to a variety of phenotypes including Leigh syndrome, a neurometabolic disorder, tumor formation, and myopathy. Recent studies show that SDH can prevent the generation of ROS (reactive oxygen species); therefore, measurement of succinate dehydrogenase activity has wide applications. BioVision’s Succinate Dehydrogenase Activity Assay kit is rapid, simple and high-throughput adaptable. In this assay, Succinate dehydrogenase converts succinate to fumarate, and transfers the electron to an artificial electron acceptor (Probe), which changes the color from blue to a colorless product (depending upon the sample enzymatic activity). This assay kit can detect less than 0.1mU Succinate Dehydrogenase Activity in a variety of samples.


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Can this assay be used for yeast SDH?
This assay can be used in a variety of sample types including yeast as long as there is succinate DH in the sample. We recommend using yeast specific lysis reagents such as http://www.biovision.com/ezlys-yeast-protein-extraction-reagent-6799.html for sample preparation. We recommend testing several different volumes of the samples to optimize the sample amount required so the numbers are within the linear range of the std curve.
Can this kit measure SDHA activity without the other components of Complex II?
We have not distinguished SDHA activity from the activity of the SDH complex while testing this kit. It measures the conversion of succinate to fumarate by SDH.
Can frozen samples be used with this assay?
Fresh samples are always preferred over frozen samples. However, frozen samples can also be used, provided, they were frozen right after isolation, were not freeze thawed multiple time (for which we recommend aliquoting the samples before freezing) and have been frozen for relatively short periods.
Is it possible to use a different wavelength than recommended for the final analysis?
It is always recommended to use the exact recommended wavelength for the most efficient results. However, most plate readers have flexibility in their band width of detection in increments of +/- 10 nm. Depending on this flexibility range, you can deviate from the recommended wavelengths within limits.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. It is recommended to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Are trial sizes of this kit available?
Unfortunately, we do not have trial sizes of this kit available. However,for customers based in the US or Canada, a 10% off list price introductory discount can be offered on the list price. For all other customers based out of this area our regional BioVision distributor should be contacted.
Can individual components of this kit be purchased separately?
Yes, any of the kit's components can be purchased separately without having to buy the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Maricela Robles-Murguia, Tissue-specific alteration of gene expression and function by RU486 and the GeneSwitch system. NPJ Aging Mech Dis, May 2019; PMC6529481.
Min Guo, et al., (2018) Ketogenic Diet Improves Brain Ischemic Tolerance and Inhibits NLRP3 Inflammasome Activation by Preventing Drp1-Mediated Mitochondrial Fission and Endoplasmic Reticulum Stress, Frontiers in Molecular Neuroscience, Mar. 2018, 29662437
Wenliang Zhang, et al., (2018) Adipose-specific lipin1 overexpression in mice protects against alcohol-induced liver injury, Scientific Reports, Jan. 2018, 29323242
For more citations of this product click here